GTX129491

antibody from GeneTex

Targeting: E2F5

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX129491

Provider

GeneTex

Product name

E2F5 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

E2F5 antibody detects E2F5 protein by western blot analysis.A. 30 £gg 293T whole cell extract B. 30 £gg whole cell extract of HA-human E2F5-transfected 293T cells10 % SDS-PAGEE2F5 antibody (GTX129491) dilution: 1:10000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Untreated (¡V) and treated (+) Mouse leukocyte extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with E2F5 antibody (GTX129491) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with E2F5 antibody (GTX129491) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Mouse tissue extract (50 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with E2F5 antibody (GTX129491) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.