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LearnAF4486
antibody from Novus Biologicals
Targeting: SIRPG
bA77C3.1, CD172g, SIRP-B2, SIRPB2, SIRPgamma
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- AF4486 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal SIRP gamma/CD172g Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human SIRP gamma/CD172g in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) SIRP alpha , rhSIRP beta 1, and recombinant mouse SIRP alpha is observed.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human SIRP gamma/CD172g by Western Blot. Western blot shows lysates of NK-92 human natural killer lymphoma cell line, Jurkat human acute T cell leukemia cell line, and human thymus tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human SIRP gamma/CD172g Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4486) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for SIRP gamma/CD172g at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of SIRP gamma/CD172g in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Sheep Anti-Human SIRP gamma/CD172g Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4486) followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # 5-001-A).
