Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 50-0369-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD36 Monoclonal Antibody (eBioNL07 (NL07)), eFluor 660, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody eBioNL07 recognizes human CD36, which is a member of the class B scavenger receptor family. CD36 was originally identified as a platelet-membrane glycoprotein also called glycoprotein IV and a receptor for thrombospondin-1 (TSP-1) and extracellular matrix proteins. Binding to TSP-1 is in the CLESH (CD36 LIMP-II Emp sequence homology) domain of CD36. CD36 expression is broad and includes microvascular (but not large vessel) endothelium, adipocytes, skeletal muscle, dendritic cells, epithelia of the retina, breast, and intestine, smooth muscle cells, and hematopoietic cells, including erythroid precursors, platelets, monocytes/macrophages, DCs and megakaryocytes. Expression on platelets is absent on Nak-a negative donors. Unlike other scavenger receptor, CD36 binds LDL that has been exposed to "minimally" oxidizing conditions. CD36 is also a fatty acid translocase (FAT) necessary for the transport of long-chain fatty acids (LCFAs) and therefore may play a role in atherosclerosis. Applications Reported: This eBioNL07 (NL07) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioNL07 (NL07) antibody has been pre-titrated and tested by flow cytometric analysis of normal human perioheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgM
- Antibody clone number
- eBioNL07 (NL07)
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Spatial and Single-Cell Transcriptional Profiling Identifies Functionally Distinct Human Dermal Fibroblast Subpopulations.
IRGM1 regulates oxidized LDL uptake by macrophage via actin-dependent receptor internalization during atherosclerosis.
Heterogeneity and immunophenotypic plasticity of malignant cells in human liposarcomas.
Terminal differentiation and loss of tumorigenicity of human cancers via pluripotency-based reprogramming.
Methionine 156 in the immunodominant domain of CD36 contributes to define the epitope recognized by the NL07 MoAb.
Platelet activation and inhibition of malarial cytoadherence by the anti-CD36 IgM monoclonal antibody NL07.
Analysis of the human CD36 leucocyte differentiation antigen by means of the monoclonal antibody NL07.
Philippeos C, Telerman SB, Oulès B, Pisco AO, Shaw TJ, Elgueta R, Lombardi G, Driskell RR, Soldin M, Lynch MD, Watt FM
The Journal of investigative dermatology 2018 Apr;138(4):811-825
The Journal of investigative dermatology 2018 Apr;138(4):811-825
IRGM1 regulates oxidized LDL uptake by macrophage via actin-dependent receptor internalization during atherosclerosis.
Xia F, Li R, Wang C, Yang S, Tian L, Dong H, Pei C, He S, Jiang P, Cheng H, Fang S, Li H, Xu H
Scientific reports 2013;3:1867
Scientific reports 2013;3:1867
Heterogeneity and immunophenotypic plasticity of malignant cells in human liposarcomas.
Zhang Y, Young ED, Bill K, Belousov R, Peng T, Lazar AJ, Pollock RE, Simmons PJ, Lev D, Kolonin MG
Stem cell research 2013 Sep;11(2):772-81
Stem cell research 2013 Sep;11(2):772-81
Terminal differentiation and loss of tumorigenicity of human cancers via pluripotency-based reprogramming.
Zhang X, Cruz FD, Terry M, Remotti F, Matushansky I
Oncogene 2013 May 2;32(18):2249-60, 2260.e1-21
Oncogene 2013 May 2;32(18):2249-60, 2260.e1-21
Methionine 156 in the immunodominant domain of CD36 contributes to define the epitope recognized by the NL07 MoAb.
Gruarin P, Ulliers D, Thorne RF, Alessio M
Molecular and cellular biochemistry 2000 Nov;214(1-2):89-95
Molecular and cellular biochemistry 2000 Nov;214(1-2):89-95
Platelet activation and inhibition of malarial cytoadherence by the anti-CD36 IgM monoclonal antibody NL07.
Alessio M, Greco NJ, Primo L, Ghigo D, Bosia A, Tandon NN, Ockenhouse CF, Jamieson GA, Malavasi F
Blood 1993 Dec 15;82(12):3637-47
Blood 1993 Dec 15;82(12):3637-47
Analysis of the human CD36 leucocyte differentiation antigen by means of the monoclonal antibody NL07.
Alessio M, Ghigo D, Garbarino G, Geuna M, Malavasi F
Cellular immunology 1991 Oct 15;137(2):487-500
Cellular immunology 1991 Oct 15;137(2):487-500
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgM Isotype Control eFluor® 660 (Product # 50-4752-82) (blue histogram) or Anti-Human CD36 eFluor® 660 (purple histogram). Cells in the monocyte gate were used for analysis.