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Western blot
Immunoelectron microscopyAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunoelectron microscopy [1]
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- Product number
- 678002 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#678002, RRID:AB_2565913
- Product name
- Purified anti-STAT3
- Antibody type
- Monoclonal
- Antigen
- Partial human STAT3 recombinant protein (621-770 a.a.) expressed in E. coli.
- Description
- STAT3 is an 88 kD member of the STAT (signal transducer and activators of transcription) protein family that is phosphorylated in response to a cytokine receptor-associated kinase activity. Phosphorylation of STAT3 induces nuclear translocation to activate transcription. STAT3 forms both homo- and heterotrimers and is involved in the activation of genes required for cell growth and apoptosis. STAT3 is also involved in gp130 signaling and binds to IL-6 response elements in various acute phase protein promoters. STAT3 is phosphorylated by signaling from IFNs, EGF, FGF, IL-5, HGF, LIF, and BMP2. STAT3 activity is inhibited by PIAS3 and GRIM-19 and can also be regulated by the Rac1 protein.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 4G4B45
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- Experimental details
- Western blot analysis of cell lysates from HeLa (lane 1), HepG2 (lane 2), and mouse thymus (lane 3) cells. Anti-STAT3 antibody (clone 4G4B45) was used, followed by HRP anti-mouse IgG antibody. β-actin antibody (clone poly6221) was used as a loading control.
- Conjugate
- Unconjugated
