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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- F48455 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- p-STAT3 Antibody (pS727)
- Antibody type
- Polyclonal
- Description
- This highly specific p-STAT3 antibody is suitable for use in Immunofluorescence/Western blot/Immunohistochemistry/ELISA applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the p-STAT3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of p-STAT3 antibody and mouse liver tissue/lysate collected before (-) or after (+) stimulation with IL-6 injection in mouse portal vein. Data is kindly provided by Drs. E. Bard-Chapeau and G-S. Feng from the Burnham Institute (La Jolla, CA).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of p-STAT3 antibody and HL-60 cells/lysate collected before (-) or after (+) stimulation with IFN-a.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent confocal image of SY5Y cells stained with p-STAT3 antibody at 1:200. Note the highly specific localization of the immunosignal to the nucleus.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human breast carcinoma tissue stained with the p-STAT3 antibody.
