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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Flow cytometry [2]
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- Product number
- R32089 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- STAT3 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific STAT3 antibody is suitable for use in Western blot/Immunofluorescence/Immunoprecipitation/Flow cytometry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the STAT3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat brain, 2) rat lung, 3) human placenta, 4) HeLa, 5) PANC and 6) mouse HEPA lysate with STAT3 antibody. Predicted/observed molecular weight ~88 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HEL, 2) human A549, 3) rat lung, 4) rat heart, 5) mouse lung and 6) mouse heart tissue lysate with STAT3 antibody. Expected molecular weight: ~92 kDa (alpha) and ~83 kDa (beta).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HEL, 2) A549, 3) HaCaT, 4) U-251, 5) K562, 6) SiHa and 7) RT4 cell lysate with STAT3 antibody. Expected molecular weight: ~92 kDa (alpha) and ~83 kDa (beta).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human A549 cells with STAT3 antibody (green) and Beta Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human U-2 OS cells with STAT3 antibody (green) and Beta Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunoprecipitation of STAT3 protein from 500ug of human HeLa whole cell lysate with 2ug of STAT3 antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human SiHa cells with STAT3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= STAT3 antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized rat PC-12 cells with STAT3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= STAT3 antibody.
