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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PA5-117700 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- OR6B3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody has been tested in direct-ELISA
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.79 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of OR6B3 using OR6B3 Polyclonal Antibody (Product # PA5-117700) at 1:2,000. Positive WB detected in: U251 whole cell lysate. Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 38 kDa. Observed band size: 38 kDa
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of OR6B3 using OR6B3 Polyclonal Antibody (Product # PA5-117700) at 1:2,000. Positive WB detected in: U251 whole cell lysate. Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 38 kDa. Observed band size: 38 kDa
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immnohistochemistry (Paraffin) analysis of OR6B3 in paraffin-embedded human skin tissue using OR6B3 Polyclonal Antibody (Product # PA5-117700) diluted at 1:300. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immnohistochemistry (Paraffin) analysis of OR6B3 in paraffin-embedded human brain tissue using OR6B3 Polyclonal Antibody (Product # PA5-117700) diluted at 1:300. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
