Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-54734 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NECAB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PGLLEEDNQW MTQINRLQKL IDRLEKKDLK LEPPEEEIIE GNTKSHIMLV QRQMSVIEED LEEFQLALKH YVE
- Concentration
- 0.08 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NECAB1 in Lane 1: Marker (kDa) 250, 130, 100, 70, 55, 35, 25, 15, 10; Lane 2: Mouse cerebral cortex tissue. Samples were probed using a NECAB1 Polyclonal Antibody (Product # PA5-54734).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NECAB1 in control (vector only transfected HEK293T lysate) and NECAB1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a NECAB1 Polyclonal Antibody (Product # PA5-54734).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of NECAB1 in human cell line A-431 shows positivity in nucleus & cytoplasm. Samples were probed using a NECAB1 Polyclonal Antibody (Product # PA5-54734).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NECAB1 in human colon using NECAB1 Polyclonal Antibody (Product # PA5-54734).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NECAB1 in human liver using NECAB1 Polyclonal Antibody (Product # PA5-54734).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NECAB1 in human cerebral cortex and pancreas tissues using NECAB1 Polyclonal Antibody (Product # PA5-54734). Corresponding NECAB1 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NECAB1 in human cerebral cortex, colon, liver and pancreas using NECAB1 Polyclonal Antibody (Product # PA5-54734) (A) shows similar protein distribution across tissues to an independent NECAB1 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NECAB1 in human cerebral cortex using NECAB1 Polyclonal Antibody (Product # PA5-54734) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NECAB1 in human pancreas using NECAB1 Polyclonal Antibody (Product # PA5-54734) shows low expression as expected.
