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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [5]
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- Product number
- PA5-54734 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NECAB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Immunogen sequence: PGLLEEDNQW MTQINRLQKL IDRLEKKDLK LEPPEEEIIE GNTKSHIMLV QRQMSVIEED LEEFQLALKH YVE Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 92%.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.10 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of NECAB1 in human cell line A-431 shows positivity in nucleus & cytoplasm. Samples were probed using a NECAB1 Polyclonal Antibody (Product # PA5-54734).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorecent analysis of NECAB1 in human cell line A-431 using NECAB1 Polyclonal Antibody (Product # PA5-54734). Staining shows localization to nucleus and cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NECAB1 in human pancreas using NECAB1 Polyclonal Antibody (Product # PA5-54734) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NECAB1 in human colon using NECAB1 Polyclonal Antibody (Product # PA5-54734).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NECAB1 in human cerebral cortex using NECAB1 Polyclonal Antibody (Product # PA5-54734) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NECAB1 in human cerebral cortex, colon, liver and pancreas using NECAB1 Polyclonal Antibody (Product # PA5-54734) (A) shows similar protein distribution across tissues to an independent NECAB1 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NECAB1 in human cerebral cortex and pancreas tissues using NECAB1 Polyclonal Antibody (Product # PA5-54734). Corresponding NECAB1 RNA-seq data are presented for the same tissues.
