PA5-55278

antibody from Invitrogen Antibodies

Targeting: NECAB1 EFCBP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-55278

Provider

Invitrogen Antibodies

Product name

NECAB1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: ASSQSGCLRI SIQKLSNESR YMIYEFWENS SVWNSHLQTN YSKTFQRSNV DFLETPELTS TMLVPASWW Highest antigen sequence identity to the following orthologs: Mouse - 97%, Rat - 97%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.05 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NECAB1 in control (vector only transfected HEK293T lysate) and NECAB1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a NECAB1 Polyclonal Antibody (Product # PA5-55278).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of NECAB1 in human cell line U-2 OS shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using a NECAB1 Polyclonal Antibody (Product # PA5-55278).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NECAB1 in human liver using NECAB1 Polyclonal Antibody (Product # PA5-55278).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NECAB1 in human colon using NECAB1 Polyclonal Antibody (Product # PA5-55278).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NECAB1 in human cerebral cortex and pancreas tissues using NECAB1 Polyclonal Antibody (Product # PA5-55278). Corresponding NECAB1 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NECAB1 in human cerebral cortex, colon, liver and pancreas using NECAB1 Polyclonal Antibody (Product # PA5-55278) (A) shows similar protein distribution across tissues to an independent NECAB1 Polyclonal Antibody (B).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NECAB1 in human cerebral cortex using NECAB1 Polyclonal Antibody (Product # PA5-55278) shows high expression.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NECAB1 in human pancreas using NECAB1 Polyclonal Antibody (Product # PA5-55278) shows low expression as expected.