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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
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- Product number
- PA5-55278 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NECAB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: ASSQSGCLRI SIQKLSNESR YMIYEFWENS SVWNSHLQTN YSKTFQRSNV DFLETPELTS TMLVPASWW
- Concentration
- 0.05 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NECAB1 in control (vector only transfected HEK293T lysate) and NECAB1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a NECAB1 Polyclonal Antibody (Product # PA5-55278).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of NECAB1 in human cell line U-2 OS shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using a NECAB1 Polyclonal Antibody (Product # PA5-55278).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NECAB1 in human liver using NECAB1 Polyclonal Antibody (Product # PA5-55278).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NECAB1 in human colon using NECAB1 Polyclonal Antibody (Product # PA5-55278).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NECAB1 in human cerebral cortex and pancreas tissues using NECAB1 Polyclonal Antibody (Product # PA5-55278). Corresponding NECAB1 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NECAB1 in human cerebral cortex, colon, liver and pancreas using NECAB1 Polyclonal Antibody (Product # PA5-55278) (A) shows similar protein distribution across tissues to an independent NECAB1 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NECAB1 in human cerebral cortex using NECAB1 Polyclonal Antibody (Product # PA5-55278) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NECAB1 in human pancreas using NECAB1 Polyclonal Antibody (Product # PA5-55278) shows low expression as expected.
