Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA502520 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502520, RRID:AB_11126038
- Product name
- NECAB1 mouse monoclonal antibody, clone OTI1H5 (formerly 1H5)
- Antibody type
- Monoclonal
- Description
- NECAB1 mouse monoclonal antibody, clone OTI1H5 (formerly 1H5)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- NECAB1
- Isotype
- IgG
- Antibody clone number
- OTI1H5
- Vial size
- 100 µl
- Concentration
- 0.18 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NECAB1 (RC209704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NECAB1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC209704 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NECAB1 antibody(TA502520), and then analyzed by flow cytometry.
- Validation comment
- FC
