V5254

antibody from NSJ Bioreagents

Targeting: NECAB1 EFCBP1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V5254

Provider

NSJ Bioreagents

Product name

NECAB1 Antibody / N-terminal EF-hand calcium binding protein 1

Antibody type

Monoclonal

Description

This highly specific NECAB1 antibody is suitable for use in Immunohistochemistry/Western blot applications with human samples.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

NECAB1/7677

Vial size

20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Aliquot the NECAB1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human brain tissue lysate with NECAB1 antibody. Predicted molecular weight ~41 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human brain tissue with NECAB1 antibody (clone NECAB1/7677). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human brain tissue with NECAB1 antibody (clone NECAB1/7677). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free NECAB1 antibody (clone NECAB1/7677) as confirmation of integrity and purity.