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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [20]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-937 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-937, RRID:AB_632229
- Product name
- Anti-PRKCD
- Antibody type
- Polyclonal (Antigen purified)
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
Submitted references Loss of protein kinase C delta gene expression in human squamous cell carcinomas: a laser capture microdissection study.
Interaction of connexin43 and protein kinase C-delta during FGF2 signaling.
The protein kinase Cdelta catalytic fragment is critical for maintenance of the G2/M DNA damage checkpoint.
Rb-dependent cellular senescence, multinucleation and susceptibility to oncogenic transformation through PKC scaffolding by SSeCKS/AKAP12.
PKC-delta and -eta, MEKK-1, MEK-6, MEK-3, and p38-delta are essential mediators of the response of normal human epidermal keratinocytes to differentiating agents.
Apigenin-induced apoptosis of leukemia cells is mediated by a bimodal and differentially regulated residue-specific phosphorylation of heat-shock protein-27.
PKCalpha expression is a marker for breast cancer aggressiveness.
Prenatal cocaine reduces AMPA receptor synaptic expression through hyperphosphorylation of the synaptic anchoring protein GRIP.
Phosphorylation of histone H3 Thr-45 is linked to apoptosis.
Differential roles of the PKC novel isoforms, PKCdelta and PKCepsilon, in mouse and human platelets.
Multiple PKCdelta tyrosine residues are required for PKCdelta-dependent activation of involucrin expression--a key role of PKCdelta-Y311.
Protein kinase C delta induces transcription of the TP53 tumor suppressor gene by controlling death-promoting factor Btf in the apoptotic response to DNA damage.
Borna disease virus blocks potentiation of presynaptic activity through inhibition of protein kinase C signaling.
Herpes simplex virus type 1 infection induces activation and recruitment of protein kinase C to the nuclear membrane and increased phosphorylation of lamin B.
Regulation of interleukin-5-induced beta2-integrin adhesion of human eosinophils by phosphoinositide 3-kinase.
Protein kinase Cdelta regulates keratinocyte death and survival by regulating activity and subcellular localization of a p38delta-extracellular signal-regulated kinase 1/2 complex.
Establishment and characterisation of a human carcinoma cell line with acquired resistance to Aplidin.
A caspase-resistant mutant of PKC-δ protects keratinocytes from UV-induced apoptosis
Protein kinase C delta blocks immediate-early gene expression in senescent cells by inactivating serum response factor.
Protein kinase Cdelta is responsible for constitutive and DNA damage-induced phosphorylation of Rad9.
Yadav V, Yanez NC, Fenton SE, Denning MF
The American journal of pathology 2010 Mar;176(3):1091-6
The American journal of pathology 2010 Mar;176(3):1091-6
Interaction of connexin43 and protein kinase C-delta during FGF2 signaling.
Niger C, Hebert C, Stains JP
BMC biochemistry 2010 Mar 25;11:14
BMC biochemistry 2010 Mar 25;11:14
The protein kinase Cdelta catalytic fragment is critical for maintenance of the G2/M DNA damage checkpoint.
LaGory EL, Sitailo LA, Denning MF
The Journal of biological chemistry 2010 Jan 15;285(3):1879-87
The Journal of biological chemistry 2010 Jan 15;285(3):1879-87
Rb-dependent cellular senescence, multinucleation and susceptibility to oncogenic transformation through PKC scaffolding by SSeCKS/AKAP12.
Akakura S, Nochajski P, Gao L, Sotomayor P, Matsui S, Gelman IH
Cell cycle (Georgetown, Tex.) 2010 Dec 1;9(23):4656-65
Cell cycle (Georgetown, Tex.) 2010 Dec 1;9(23):4656-65
PKC-delta and -eta, MEKK-1, MEK-6, MEK-3, and p38-delta are essential mediators of the response of normal human epidermal keratinocytes to differentiating agents.
Adhikary G, Chew YC, Reece EA, Eckert RL
The Journal of investigative dermatology 2010 Aug;130(8):2017-30
The Journal of investigative dermatology 2010 Aug;130(8):2017-30
Apigenin-induced apoptosis of leukemia cells is mediated by a bimodal and differentially regulated residue-specific phosphorylation of heat-shock protein-27.
Gonzalez-Mejia ME, Voss OH, Murnan EJ, Doseff AI
Cell death & disease 2010 Aug 19;1(8):e64
Cell death & disease 2010 Aug 19;1(8):e64
PKCalpha expression is a marker for breast cancer aggressiveness.
Lønne GK, Cornmark L, Zahirovic IO, Landberg G, Jirström K, Larsson C
Molecular cancer 2010 Apr 14;9:76
Molecular cancer 2010 Apr 14;9:76
Prenatal cocaine reduces AMPA receptor synaptic expression through hyperphosphorylation of the synaptic anchoring protein GRIP.
Bakshi K, Gennaro S, Chan CY, Kosciuk M, Liu J, Stucky A, Trenkner E, Friedman E, Nagele RG, Wang HY
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 May 13;29(19):6308-19
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 May 13;29(19):6308-19
Phosphorylation of histone H3 Thr-45 is linked to apoptosis.
Hurd PJ, Bannister AJ, Halls K, Dawson MA, Vermeulen M, Olsen JV, Ismail H, Somers J, Mann M, Owen-Hughes T, Gout I, Kouzarides T
The Journal of biological chemistry 2009 Jun 12;284(24):16575-16583
The Journal of biological chemistry 2009 Jun 12;284(24):16575-16583
Differential roles of the PKC novel isoforms, PKCdelta and PKCepsilon, in mouse and human platelets.
Pears CJ, Thornber K, Auger JM, Hughes CE, Grygielska B, Protty MB, Pearce AC, Watson SP
PloS one 2008;3(11):e3793
PloS one 2008;3(11):e3793
Multiple PKCdelta tyrosine residues are required for PKCdelta-dependent activation of involucrin expression--a key role of PKCdelta-Y311.
Zhu L, Brodie C, Balasubramanian S, Eckert RL
The Journal of investigative dermatology 2008 Apr;128(4):833-45
The Journal of investigative dermatology 2008 Apr;128(4):833-45
Protein kinase C delta induces transcription of the TP53 tumor suppressor gene by controlling death-promoting factor Btf in the apoptotic response to DNA damage.
Liu H, Lu ZG, Miki Y, Yoshida K
Molecular and cellular biology 2007 Dec;27(24):8480-91
Molecular and cellular biology 2007 Dec;27(24):8480-91
Borna disease virus blocks potentiation of presynaptic activity through inhibition of protein kinase C signaling.
Volmer R, Monnet C, Gonzalez-Dunia D
PLoS pathogens 2006 Mar;2(3):e19
PLoS pathogens 2006 Mar;2(3):e19
Herpes simplex virus type 1 infection induces activation and recruitment of protein kinase C to the nuclear membrane and increased phosphorylation of lamin B.
Park R, Baines JD
Journal of virology 2006 Jan;80(1):494-504
Journal of virology 2006 Jan;80(1):494-504
Regulation of interleukin-5-induced beta2-integrin adhesion of human eosinophils by phosphoinositide 3-kinase.
Sano M, Leff AR, Myou S, Boetticher E, Meliton AY, Learoyd J, Lambertino AT, Munoz NM, Zhu X
American journal of respiratory cell and molecular biology 2005 Jul;33(1):65-70
American journal of respiratory cell and molecular biology 2005 Jul;33(1):65-70
Protein kinase Cdelta regulates keratinocyte death and survival by regulating activity and subcellular localization of a p38delta-extracellular signal-regulated kinase 1/2 complex.
Efimova T, Broome AM, Eckert RL
Molecular and cellular biology 2004 Sep;24(18):8167-83
Molecular and cellular biology 2004 Sep;24(18):8167-83
Establishment and characterisation of a human carcinoma cell line with acquired resistance to Aplidin.
Losada A, López-Oliva JM, Sánchez-Puelles JM, García-Fernández LF
British journal of cancer 2004 Oct 4;91(7):1405-13
British journal of cancer 2004 Oct 4;91(7):1405-13
A caspase-resistant mutant of PKC-δ protects keratinocytes from UV-induced apoptosis
A M D'Costa, M F Denning
Cell Death and Differentiation 2004 Dec;12(3):224-232
Cell Death and Differentiation 2004 Dec;12(3):224-232
Protein kinase C delta blocks immediate-early gene expression in senescent cells by inactivating serum response factor.
Wheaton K, Riabowol K
Molecular and cellular biology 2004 Aug;24(16):7298-311
Molecular and cellular biology 2004 Aug;24(16):7298-311
Protein kinase Cdelta is responsible for constitutive and DNA damage-induced phosphorylation of Rad9.
Yoshida K, Wang HG, Miki Y, Kufe D
The EMBO journal 2003 Mar 17;22(6):1431-41
The EMBO journal 2003 Mar 17;22(6):1431-41
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 229, 107, 78, 46, 31, 26, 16.2
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 78
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in ,reaction center cells.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
