PA5-87443

antibody from Invitrogen Antibodies

Targeting: PRKCD

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-87443

Provider

Invitrogen Antibodies

Product name

PKC delta Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MAPFLRIAFN SYELGSLQAE DEANQPFCAV KMKEALSTER GKTLVQKKPT MYPEWKSTFD AHIYEGRVIQ IVLMRAAEEP VSEVTVGVSV LAERCKKNNG KAEFWLDLQP QAKVLMSVQY FLEDVDCKQS MRSEDEAKFP TMNRRGAIKQ AKIHYIKNHE Positive Samples: MCF7, Mouse testis; Cellular Location: Cell membrane, Cytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleus, Peripheral membrane protein, perinuclear region

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.12 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Targeting Cutaneous T-Cell Lymphoma Cells by Ingenol Mebutate (PEP005) Correlates with PKCδ Activation, ROS Induction as Well as Downregulation of XIAP and c-FLIP.

Sumarni U, Reidel U, Eberle J
Cells 2021 Apr 23;10(5)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of PKC delta was performed in NIH/3T3 cells using PKC delta Polyclonal Antibody (Product # PA5-87443).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PKC delta in paraffin-embedded rat lung. Samples were incubated with PKC delta Polyclonal antibody (Product # PA5-87443) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PKC delta in paraffin-embedded human tonsil. Samples were incubated with PKC delta Polyclonal antibody (Product # PA5-87443) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PKC delta in paraffin-embedded mouse testis. Samples were incubated with PKC delta Polyclonal antibody (Product # PA5-87443) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 Role of PKCdelta in PEP005-induced apoptosis. ( a ) Effects of PEP005 (50 nM, 24 h) on PKCdelta proform (78 kDa) were investigated in four CTCL cell lines. ( b , c ) Lacking effects of QVD-Oph (QVD, 5 uM, b) and vitamin E (VitE, 1 mM, c ) on PEP005-induced downregulation of PKCdelta proform are shown (50 nM, 24 h). ( d , e ) Inhibition of PEP005-induced apoptosis ( d ) and restoration of cell viability ( e ) by Bis-1 in HH and HuT-78. Cells were treated for 24 h with PEP005 (50 nM) and/or Bis-1 (HH, 1 uM; HuT-78, 0.25 uM). ( f ) Inhibition of PEP005-mediated caspase-3, -8, and -9 processing through Bis-1, as investigated by Western blotting in HH and HuT-78. Cells were treated for 24 h with 50 nM PEP005; Bis-1 was used at 1 (HH) and 0.25 uM (HuT-78), respectively). ( g , h ) Antagonistic effects of Bis-1 on PEP005-mediated loss of MMP ( g ) and on PEP005-induced ROS production ( h ) in cell line HuT-78 (Time: 24 h; PEP005: 50 nM; Bis-1: 0.25 uM). ( a - c , f ) For Western blotting, 30 ug of each protein extract was loaded per lane, and blots were probed with antibodies for PKCdelta proform (78 kDa), cleaved caspase-3 (21, 19, 17 kDa), caspase-8 (proform, 57 kDa; cleavage products, 43/41 kDa) and caspase-9 (cleavage product, 35 kDa). GAPDH (37 kDa) was used as loading control. For Western blots, two independent series of protein extracts revealed highly comparable results. ( d , e , g , h ) Mean values of triplicates +- SDs of representative experiments are shown. At