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- Antigen structure
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- Product number
- PA5-17552 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PKC delta Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Concentration
- 32 µg/mL
Submitted references Transcriptional Profiling of Primate Central Nucleus of the Amygdala Neurons to Understand the Molecular Underpinnings of Early-Life Anxious Temperament.
Kovner R, Souaiaia T, Fox AS, French DA, Goss CE, Roseboom PH, Oler JA, Riedel MK, Fekete EM, Fudge JL, Knowles JA, Kalin NH
Biological psychiatry 2020 Oct 15;88(8):638-648
Biological psychiatry 2020 Oct 15;88(8):638-648
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PKCdelta in extracts COS and C6 cells using PKC-delta polyclonal antibody (Product # PA5-17552).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PKCdelta in Baculovirus expressed PKC isoforms using PKC-delta polyclonal antibody (Product # PA5-17552) demonstrating the isoform-specificity of PKC-delta antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using anti-PKC delta Monoclonal Antibody (Product # PA5-17552) and a 77 kDa band corresponding to PKC delta was observed across cell lines and tissues tested except in Mouse, Rat Heart and Mouse, Rat Liver. Whole cell Extracts (30 µg lysate) of Raji (Lane 1), tissue extracts of Mouse Brain (Lane 2), Rat Brain (Lane 3), Mouse Spleen (Lane 4), Mouse Heart (Lane 5), Rat Heart (Lane 6), Mouse Liver (Lane 7) and Rat Liver (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
