GTX25658

antibody from GeneTex

Targeting: PRKCD

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

GTX25658

Provider

GeneTex

Proper citation

GeneTex Cat#GTX25658, RRID:AB_380629

Product name

PKC delta (phospho Ser664) antibody

Antibody type

Polyclonal

Reactivity

Human, Rat

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Lysates prepared from K562 cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-12), or treated with lambda phosphatase (13), blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with PKCdelta [pS664] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 13), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphoserine-containing peptide (3), the phosphopeptide immunogen (4), or the phosphopeptides corresponding to other PKC isoforms (5-12). After washing, membranes were incubated with goat F(ab)2 anti-rabbit IgG HRP conjugate and bands were detected. The data show that the peptide corresponding to PKCdelta [pS664] blocks the antibody signal. The peptide corresponding to PKCalpha [pS657] partially blocks the antibody signal, while those for PKCbetaII [pS660], and rat PKCdelta [pS662] block the antibody signal more thoroughly, indicating their respective reactivity with this antibody. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific