Antibody data
- Product number
- PA5-17834
- Provider
- Invitrogen Antibodies
- Product name
- Anti-VAV1 Polyclonal Antibody
- Provider product page
- Invitrogen Antibodies - PA5-17834
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody. This antibody is not cross-reactive with Vav2, Vav3 or CelVav.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Lot Dependent
- Storage
- -20°C
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-VAV1 Polyclonal Antibody (Product # PA5-17834) and a 98kDa band corresponding to VAV1 was observed in Raji, MOLT-4, Jurkat, THP-1, Mouse Thymus but not in T-47D, MCF7 and Mouse Kidney. Whole cell extracts (30ug) of Raji (Lane 1), MOLT-4 (Lane 2), Jurkat (Lane 3), THP-1 (Lane 4), T-47D (Lane 5) and MCF7 (Lane 6), and Tissue extracts (30ug) of Mouse Thymus (Lane 7) and Mouse Kidney (Lane 8) were electrophoresed using NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (4ug/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Vav1 in untreated Jurkat cells using a Vav1 polyclonal antibody (Product # PA5-17834) (blue) compared to a nonspecific negative control antibody (red).
Supportive data in Antibodypedia
