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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX100279 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100279, RRID:AB_1240819
- Product name
- Flotillin 2 antibody [C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flotillin 2 antibody [C3], C-term detects FLOT2 protein by western blot analysis.A. 50 ?g mouse brain lysate/extract 10% SDS-PAGEFlotillin 2 antibody [C3], C-term (GTX100279) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flotillin 2 antibody [C3], C-term detects FLOT2 protein by western blot analysis.A. 50 ?g rat brain lysate/extract 10% SDS-PAGEFlotillin 2 antibody [C3], C-term (GTX100279) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 ?g of whole cell lysate)A:HeLa S3(GTX14654)10% SDS PAGEGTX100279 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flotillin 2 antibody detects Flotillin 2 protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 10% SDS-PAGE, and blotted with Flotillin 2 antibody (GTX100279) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLaS3, using Flotillin-2 (GTX100279) antibody at 1:100 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of Flotillin 2 protein from HeLa whole cell extracts using 5 £gg of Flotillin 2 antibody [C3], C-term (GTX100279).Western blot analysis was performed using Flotillin 2 antibody [C3], C-term (GTX100279).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung cancer, using Flotillin-2 (GTX100279) antibody at 1:100 dilution.
