GTX114411

antibody from GeneTex

Targeting: FLOT2 ECS-1, ECS1, ESA, ESA1, M17S1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX114411

Provider

GeneTex

Proper citation

GeneTex Cat#GTX114411, RRID:AB_10619212

Product name

Flotillin 2 antibody [N1C1]

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

References

Submitted references

In vivo quantitative proteomics of somatosensory cortical synapses shows which protein levels are modulated by sensory deprivation.

Butko MT, Savas JN, Friedman B, Delahunty C, Ebner F, Yates JR 3rd, Tsien RY
Proceedings of the National Academy of Sciences of the United States of America 2013 Feb 19;110(8):E726-35

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE GTX114411 diluted at 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: HCT116 10% SDS PAGE Flotillin-2 antibody GTX114411 diluted at 1:1000

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Flotillin 2 antibody [N1C1] immunoprecipitates Flotillin 2 protein in IP experiments.IP samples: HCT-116 whole cell extractA. Control with 4 £gg of preimmune Rabbit IgGB. Immunoprecipitation of Flotillin 2 protein by 4 £gg Flotillin 2 antibody [N1C1] (GTX114411)10 % SDS-PAGEThe immunoprecipitated Flotillin 2 protein was detected by Flotillin 2 antibody [N1C1] (GTX114411) diluted at 1:1000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using Flotillin 2(GTX114411) antibody at 1:100 dilution.