Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [6]
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Validation data
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- Product number
- LS-C407836 - Provider product page
- Provider
- LSBio
- Product name
- FLOT2 / Flotillin 2 Antibody (aa169-344) LS-C407836
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Storage
- At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Flotillin 2 antibody Western blot. All lanes: Anti Flotillin 2 at 0.5 ug/ml. Lane 1: Rat Liver Tissue Lysate at 50 ug. Lane 2: Rat Lung Tissue Lysate at 50 ug. Lane 3: Mouse Liver Tissue Lysate at 50 ug. Lane 4: Mouse Lung Tissue Lysate at 50 ug. Lane 5: Mouse Testis Tissue Lysate at 50 ug. Lane 6: Human Placenta Tissue Lysate at 50 ug. Lane 7: A375 Whole Cell Lysate at 40 ug. Lane 8: HELA Whole Cell Lysate at 40 ug. Lane 9: 22RV1 Whole Cell Lysate at 40 ug. Lane 10: NIH3T3 Whole Cell Lysate at 40 ug. Predicted band size: 47 kD. Observed band size: 47 kD.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Flotillin 2 antibody IHC-paraffin. IHC(P): Mouse Intestine Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Flotillin 2 antibody IHC-paraffin. IHC(P): Rat Kidney Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Flotillin 2 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody. Flotillin 2 was detected in frozen section of mouse kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Flotillin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody. Flotillin 2 was detected in frozen section of rat kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Flotillin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody. Flotillin 2 was detected in frozen section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Flotillin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.