LS-C407836

antibody from LSBio

Targeting: FLOT2 ECS-1, ECS1, ESA, ESA1, M17S1

Western blot

Antibody data

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Validation data

Product number: LS-C407836 - Provider product page
Provider: LSBio
Product name: FLOT2 / Flotillin 2 Antibody (aa169-344) LS-C407836
Antibody type: Polyclonal
Description: Immunogen affinity purified
Reactivity: Human, Mouse, Rat
Host: Rabbit
Storage: At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

FLOT2 protein structure - LS-C407836 shown in red.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Flotillin 2 antibody IHC-paraffin. IHC(P): Mouse Intestine Tissue.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Flotillin 2 antibody IHC-paraffin. IHC(P): Rat Kidney Tissue.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Flotillin 2 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody. Flotillin 2 was detected in frozen section of mouse kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Flotillin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody. Flotillin 2 was detected in frozen section of rat kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Flotillin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody. Flotillin 2 was detected in frozen section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Flotillin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.