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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [6]
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- Product number
- R32227 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Flotillin 2 Antibody
- Antibody type
- Polyclonal
- Antigen
- Amino acids 169-344 of human Flotillin 2 were used as the immunogen for the Flotillin 2 antibody.
- Description
- Antigen affinity
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the Flotillin 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat liver, 2) rat lung, 3) mouse liver, 4) mouse lung, 5) mouse testis, 6) human placenta, 7) A375, 8) HeLa, 9) 22RV1 and 10) mouse NIH3T3 lysate with Flotillin 2 antibody. Predicted molecular weight: ~47 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human breast cancer tissue with Flotillin-2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE mouse intestine with Flotillin-2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE rat kidney with Flotillin-2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of frozen mouse kidney tissue with Flotillin 2 antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of frozen rat kidney tissue with Flotillin 2 antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of frozen rat small intestine tissue with Flotillin 2 antibody.
