NBP1-30881

antibody from Novus Biologicals

Targeting: FLOT2 ECS-1, ECS1, ESA, ESA1, M17S1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-30881

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-30881, RRID:AB_2247202

Product name

Rabbit Polyclonal Flotillin 2 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Flotillin 2 Antibody [NBP1-30881] - 50 ug mouse brain lysate/extract. Antibody dilution 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Flotillin 2 Antibody [NBP1-30881] - 50 ug rat brain lysate/extract. Antibody dilution 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Flotillin 2 Antibody [NBP1-30881] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and blotted with Flotillin 2 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Flotillin 2 Antibody [NBP1-30881] - 293T whole cell extracts and 293T exosome extract (2.6 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 2 antibody [C3], C-term (NBP1-30881). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: Flotillin 2 Antibody [NBP1-30881] - Immunoprecipitation of Flotillin 2 protein from HeLa whole cell extracts using 5 ug of Flotillin 2 antibody [C3], C-term. Western blot analysis was performed using Flotillin 2 antibody [C3], C-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Flotillin 2 Antibody [NBP1-30881] - Paraffin-embedded Lung CA, using antibody at 1:100 dilution.