PA5-17178

antibody from Invitrogen Antibodies

Targeting: FLOT2 ECS-1, ECS1, ESA, ESA1, M17S1

Antibody data

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Validation data

FLOT2 protein structure - PA5-17178 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Flotillin-2 in extracts from 293 and NIH/3T3 cells using Flotillin-2 polyclonal antibody (Product # PA5-17178).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of FLOT2 was achieved by transfecting HeLa with FLOT2 specific siRNAs (Silencer® select Product # s5284, s5285). Western blot analysis (Fig. a) was performed using whole cell extracts from the FLOT2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Flotillin 2 Polyclonal Antibody (Product # PA5-17178, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to FLOT2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Flotillin 2 Polyclonal Antibody (Product # PA5-17178) and a 53 kDa band corresponding to FLOT2 was observed in HeLa, A-431, NIH/3T3 and Mouse Brain along with an uncharacterized band (*) at ~80 kDa in A-431. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A-431 (Lane 2), Molt-4 (Lane 3), NIH/3T3 (Lane 4) and tissue extracts (30 µg lysate) of Mouse Brain (Lane 5) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX) Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).