Antibody data
Product number STJ29590 - Provider product page
Provider St John's Laboratory
Product name Anti-TEAD3 antibody (130-330) (STJ29590) Antibody type Polyclonal Description Rabbit polyclonal antibody anti-TEAD3 (130-330) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Reactivity Human, Mouse, Rat Host Rabbit Conjugate Unconjugated Antigen sequence NA
Epitope NA Isotype IgG Antibody clone number NA Vial size NA Concentration NA Storage Store in a freezer at-20°C and avoid freeze-thaw cycles. Handling NA
TEAD3 protein structure - STJ29590 shown in red.
Supportive validation
Submitted by
St John's Laboratory (provider)
Main image
Experimental details
Western blot analysis of extracts of various cell lines, using TEAD3 antibody (STJ29590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Sample type
NA
Validation comment
NA
Primary Ab dilution
NA
Other comments
NA
Secondary Ab
NA
Secondary Ab dilution
NA
Protocol
NA
Appendix
NA
Show more
Supportive validation
Submitted by
St John's Laboratory (provider)
Main image
Experimental details
Immunofluorescence analysis of A549 cells using TEAD3 antibody (STJ29590).
Sample type
NA
Validation comment
NA
Primary Ab dilution
NA
Other comments
NA
Secondary Ab
NA
Secondary Ab dilution
NA
Protocol
NA
Appendix
NA
Show more
Supportive validation
Submitted by
St John's Laboratory (provider)
Main image
Experimental details
Confocal immunofluorescence analysis of U-2 OS cells using TEAD3 Polyclonal Antibody (STJ29590) at dilution of 1:400. Blue: DAPI for nuclear staining.
Sample type
NA
Validation comment
NA
Primary Ab dilution
NA
Other comments
NA
Secondary Ab
NA
Secondary Ab dilution
NA
Protocol
NA
Appendix
NA
Show more
Supportive validation
Submitted by
St John's Laboratory (provider)
Main image
Experimental details
Immunohistochemistry of paraffin-embedded human placenta using TEAD3 rabbit polyclonal antibody (STJ29590) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Sample type
NA
Validation comment
NA
Primary Ab dilution
NA
Other comments
NA
Secondary Ab
NA
Secondary Ab dilution
NA
Protocol
NA
Appendix
NA
Show more