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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Other assay [1]
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- Product number
- PA5-113653 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TEF5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Immunogen sequence: MASNSWNASS SPGEAREDGP EGLDKGLDND AEGVWSPDIE QSFQEALAIY For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. Predicted homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 93%; Rat: 100%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot of HepG2, Human stomach using a Polyclonal TEF5 Antibody (Product # PA5-113653). Positive control (+): HepG2 (HG). Negative control (-): Human stomach (ST). Antibody concentration: 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot of Human heart using a Polyclonal TEF5 Antibody (Product # PA5-113653).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot of Human HepG2 using a Polyclonal TEF5 Antibody (Product # PA5-113653). Antibody Dilution: 1.0 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of HEK293 Whole Cell Lysate using a TEF5 Polyclonal Antibody (Product # PA5-113653). TEF5 was immunoprecipitated from 2 mg HEK293 Whole Cell Lysate with PA5-113653 diluted 1:200. Western blot was performed using PA5-113653 diluted 1:1,000. Lane 1: Control IP in HEK293 Whole Cell Lysate. Lane 2: TEF5 IP with PA5-113653 in HEK293 Whole Cell Lysate. Lane 3: Input of HEK293 Whole Cell Lysate.
