PA5-96439

antibody from Invitrogen Antibodies

Targeting: PDYN ADCA, PENKB, SCA23

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96439

Provider

Invitrogen Antibodies

Product name

Prodynorphin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: DCLSRCSLCA VKTQDGPKPI NPLICSLQCQ AALLPSEEWE RCQSFLSFFT PSTLGLNDKE DLGSKSVGEG PYSELAKLSG SFLKELEKSK FLPSISTKEN TLSKSLEEKL RGLSDGFREG AESELMRDAQ LNDGAMETGT LYLAEEDPKE QVKRYGGFLR KYPKRSSEVA GEGDGDSMGH EDLYKRYGGF LRRIRPKLKW DNQKRYGGFL RRQFKVVTRS QEDPNAYSGE LFDA; Positive Samples: U-251MG, Mouse liver, Mouse pancreas

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.92 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Adjusting vascular permeability, leukocyte infiltration, and microglial cell activation to rescue dopaminergic neurons in rodent models of Parkinson's disease.

Cai HY, Fu XX, Jiang H, Han S
NPJ Parkinson's disease 2021 Oct 8;7(1):91

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Optogenetic activation of striatal D1R and D2R cells differentially engages downstream connected areas beyond the basal ganglia.

Grimm C, Frässle S, Steger C, von Ziegler L, Sturman O, Shemesh N, Peleg-Raibstein D, Burdakov D, Bohacek J, Stephan KE, Razansky D, Wenderoth N, Zerbi V
Cell reports 2021 Dec 28;37(13):110161

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Prodynorphin in extracts of various cell lines using Prodynorphin Polyclonal Antibody (Product # PA5-96439) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Prodynorphin in paraffin-embedded rat brain using Prodynorphin Polyclonal Antibody (Product # PA5-96439) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Prodynorphin in paraffin-embedded human lung cancer using Prodynorphin Polyclonal Antibody (Product # PA5-96439) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Prodynorphin in paraffin-embedded human gastric cancer using Prodynorphin Polyclonal Antibody (Product # PA5-96439) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Prodynorphin in paraffin-embedded mouse brain using Prodynorphin Polyclonal Antibody (Product # PA5-96439) at a dilution of 1:200.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 15 C16 in combination with Ang-1 treatment decreased the expression of phospho-DARPP-32, and increased the expression of pdyn and PPE. Western blot analysis showed no significant difference in the expression of DARPP-32 among the different groups ( a , b ). The expression of phospho-DARPP-32 in the vehicle-treated group was higher than that in normal controls ( c , d ). The combination of C16 and Ang-1 inhibited the upregulation of phospho-DARPP-32 in model animals. The downregulation of prodynorphin (pdyn, e , f ) and the upregulation of preproenkephalin (PPE, g , h ) in the model groups were remarkably reversed by treatment with C16, Ang-1, and C16 plus Ang-1. a p < 0.05 versus the normal control group; b p < 0.05 versus the vehicle group ( c ), p < 0.05 versus the C16-treated group ( d ), p < 0.05 versus the Ang-1-treated group. Darpp-32 protein: 34 kDa; pDarpp-32 protein: 34 kDa; Pdyn protein: 28 kDa; PPE protein: 30 kDa; beta-actin protein: 42 kDa; Abbreviations: DARPP, dopamine- and cAMP-regulated neuronal phosphoprotein; pdyn, prodynorphin; PPE, preproenkephalin; C16, peptide (KAFDITYVRLKF) that can selectively bind integrin alpha nu beta 3 ; Ang-1, angiopoietin-1.