Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-22286 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Prodynorphin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2, Molt-4, Raji, mouse brain, rat brain.
- Concentration
- 0.29 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Prodynorphin was performed by separating 50 µg of various tissue extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a Prodynorphin Polyclonal Antibody (Product # PA5-22286) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Prodynorphin Polyclonal Antibody (Product # PA5-22286). Sample (30 µg of whole cell lysate). Lane A: Molt-4. Lane B: Raji. 12% SDS PAGE. Prodynorphin Polyclonal Antibody (Product # PA5-22286) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of Prodynorphin was performed in frozen-sectioned mouse hippocampus tissue using Prodynorphin Polyclonal Antibody (Product # PA5-22286) at a dilution of 1:250 (Green). Blue: Fluoroshield with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PDYN (Product # PA5-22286) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
