PA5-64309

antibody from Invitrogen Antibodies

Targeting: DNAH2 DNHD3, FLJ46675, KIAA1503

Provider product page for PA5-64309

Immunocytochemistry

Immunohistochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [1]
Comments [0]
Validations
Immunocytochemistry [1]
Other assay [2]

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Product number: PA5-64309 - Provider product page
Provider: Invitrogen Antibodies
Product name: DNAH2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Immunogen sequence: PGEWENACNE MQRMLIVRSL RQDRVAFCVT SFIITNLGSR FIEPPVLNMK SVLEDSTPRS PLVFILSPGV DPTSALL Highest antigen sequence identity to the following orthologs: Mouse - 96%, Rat - 95%.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

DNAH2 protein structure - PA5-64309 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 2 DNAH2 deficiency in mice recapitulates multiple morphological abnormalities of the flagella in humans. (A) Confocal images of DNAH2 in WT and Dnah2 Delta/Delta sperm. DNAH2 were immunodetected from WT sperm but not from Dnah2 Delta/Delta sperm. Sperm heads were counterstained with Hoechst. Shown images are confocal images merged to the corresponding DIC images. H, head; MP, midpiece; PP, principal piece. (B) Testicular and epididymal histology of Dnah2 Delta/Delta mouse testis and epididymis. H/E stained sections show Dnah2 Delta/Delta males produce less sperm with elongated tails in the lumen of seminiferous tubule in testis ( top ), resulting in fewer sperm cells in the epididymis ( bottom , corpus). (C,D) Multiple morphological defects in epididymal Dnah2 Delta/Delta sperm. (C) Morphology of Dnah2 +/Delta ( left ) and Dnah2 Delta/Delta ( right ) sperm. Sperm from heterozygous males are morphologically normal. Dnah2 Delta/Delta sperm, however, present MMAF-like phenotypes, such as absent, short, coiled, and irregular-caliber flagella (from left top , clockwise direction). Sperm heads were Hoechst-stained (blue). Fluorescence confocal and corresponding DIC images are merged. (D) Proportions of sperm with defective flagellar morphologies. Sperm with absent, short, bent, coiled, and irregular-caliber flagella were quantified from WT (gray) and Dnah2 Delta/Delta (red) male mice ( N = 3, each). Sperm proportions of each pattern were statistically compared between WT and

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 3 Flagellar ultrastructure is disorganized, and axonemal proteins express abnormally in Dnah2 - sperm. (A,B) Scanning electron microscopy images of Dnah2 +/Delta (A) and Dnah2 Delta/Delta (B) sperm. Dnah2 Delta/Delta sperm display absence of ( left ) or an irregular ( right ) mitochondrial helical sheath in the midpiece of the sperm tail, along with abnormal head shapes. Arrowheads indicate annulus. H, head; MP, midpiece; and PP, principal piece. (C,D) Transmission electron microscopy images of Dnah2 +/Delta (C) and Dnah2 Delta/Delta (D) sperm. Representative cross-section images of TEM reveal conformational defects in the midpiece of Dnah2 Delta/Delta sperm. Incomplete 9+2 axonemal structure ( left ), delocalized outer dense fibers (ODF, arrow) and microtubule doublet ( middle , arrowhead), and lack of the axonemal components ( right ) were observed in the Dnah2 Delta/Delta sperm. Inner (yellow asterisk) and outer (white asterisk) dynein arm structure were observed from microtubule doublet occasionally ( left , inset; scale bar = 50 nm). Microtubule doublets are numbered with the absence of the corresponding microtubule doublet in red. M, mitochondria. (E) Confocal fluorescence images of axonemal proteins in WT and Dnah2 Delta/Delta sperm. Dnah2 Delta/Delta sperm do not express another IDA component (DNAH1, magenta) and a radial spoke protein (RSPH3, green) but an ODA protein (DNAH9, yellow). Flagella microtubules (AcTub, white) and sperm heads (Hoechst, blue) ser