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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-47472 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- H-cadherin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, approximately 100% cross-reactivity with recombinant mouse Cadherin-13 is observed, and less than 10% cross-reactivity with recombinant human (rh) N-Cadherin is observed, and less than 2% cross-reactivity with rhCadherin-8, rhCadherin-11, rhCadherin-17, rhE-Cadherin, rhP-Cadherin, rhVE-Cadherin and rhR-Cadherin is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of human brain (cortex) tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-human Cadherin-13 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47472) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Cadherin-13 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of human brain (motor cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for Cadherin-13 at approximately 114 kDa (as indicated) using 10 µg/mL of Goat Anti-human Cadherin-13 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47472) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody. This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Cadherin-13 was detected in immersion fixed NCI-H460 human large cell lung carcinoma cell line using 10 µg/mL Goat Anti-human Cadherin-13 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47472) for 3 hours at room temperature. Cells were stained with the 557-conjugated Anti-Goat IgG Secondary Antibody (re and counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Cadherin-13 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-human Cadherin-13 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47472) at 0.1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB (brown and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of keratinocytes.
