PA5-47472

antibody from Invitrogen Antibodies

Targeting: CDH13 CDHH

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47472

Provider

Invitrogen Antibodies

Product name

H-cadherin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, approximately 100% cross-reactivity with recombinant mouse Cadherin-13 is observed, and less than 10% cross-reactivity with recombinant human (rh) N-Cadherin is observed, and less than 2% cross-reactivity with rhCadherin-8, rhCadherin-11, rhCadherin-17, rhE-Cadherin, rhP-Cadherin, rhVE-Cadherin and rhR-Cadherin is observed.

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of human brain (cortex) tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-human Cadherin-13 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47472) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Cadherin-13 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of human brain (motor cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for Cadherin-13 at approximately 114 kDa (as indicated) using 10 µg/mL of Goat Anti-human Cadherin-13 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47472) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody. This experiment was conducted under reducing conditions.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Cadherin-13 was detected in immersion fixed NCI-H460 human large cell lung carcinoma cell line using 10 µg/mL Goat Anti-human Cadherin-13 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47472) for 3 hours at room temperature. Cells were stained with the 557-conjugated Anti-Goat IgG Secondary Antibody (re and counterstained with DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Cadherin-13 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-human Cadherin-13 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47472) at 0.1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB (brown and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of keratinocytes.