Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF6394 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal EGLN2/PHD1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human EGLN2/PHD1 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Ginsenoside 20(S)-Rg3 suppresses ovarian cancer migration via hypoxia-inducible factor 1 alpha and nuclear factor-kappa B signals.
Docetaxel induced-JNK2/PHD1 signaling pathway increases degradation of HIF-1α and causes cancer cell death under hypoxia.
NQO1 inhibits proteasome-mediated degradation of HIF-1α.
Ginsenoside 20(S)-Rg3 targets HIF-1α to block hypoxia-induced epithelial-mesenchymal transition in ovarian cancer cells.
Liu T, Zhao L, Hou H, Ding L, Chen W, Li X
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2017 May;39(5):1010428317692225
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2017 May;39(5):1010428317692225
Docetaxel induced-JNK2/PHD1 signaling pathway increases degradation of HIF-1α and causes cancer cell death under hypoxia.
Oh ET, Kim CW, Kim SJ, Lee JS, Hong SS, Park HJ
Scientific reports 2016 Jun 6;6:27382
Scientific reports 2016 Jun 6;6:27382
NQO1 inhibits proteasome-mediated degradation of HIF-1α.
Oh ET, Kim JW, Kim JM, Kim SJ, Lee JS, Hong SS, Goodwin J, Ruthenborg RJ, Jung MG, Lee HJ, Lee CH, Park ES, Kim C, Park HJ
Nature communications 2016 Dec 14;7:13593
Nature communications 2016 Dec 14;7:13593
Ginsenoside 20(S)-Rg3 targets HIF-1α to block hypoxia-induced epithelial-mesenchymal transition in ovarian cancer cells.
Liu T, Zhao L, Zhang Y, Chen W, Liu D, Hou H, Ding L, Li X
PloS one 2014;9(9):e103887
PloS one 2014;9(9):e103887
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human EGLN2/PHD1 by Western Blot. Western blot shows lysates of MDA-MB-231 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Human EGLN2/PHD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6394) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EGLN2/PHD1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.