LS-C181966

antibody from LSBio

Targeting: APP AD1

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunocytochemistry (Enhanced validation)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C181966

Provider

LSBio

Product name

APP / Beta Amyloid Precursor Antibody (clone MOAB-2, Biotin) LS-C181966

Antibody type

Monoclonal

Description

Protein G purified

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Biotin

Isotype

IgG

Antibody clone number

MOAB-2

Storage

Store at 4°C in the dark.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot: beta Amyloid Antibody (MOAB-2) - Western blot of beta Amyloid (MOAB-2) antibody in 1) 100 pmole beta Amyloid 42, 2) 5xFAD mouse brain homogenate Repetition 1 and 3) 5xFAD mouse brain homogenate Repetition 2. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot: beta Amyloid Antibody (MOAB-2) - Western blot of cell lysates from HEK-APP SWE/BACE1 cells probed with an antibody against the C-terminus of APP (Lanes 1 and 2) and beta Amyloid (MOAB-2, Lanes 3 and 4). Beta Amyloid (MOAB-2) does not detect APP (from PMID: 22423893). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: beta Amyloid Antibody (MOAB-2) - Immunofluorescent detection of beta Amyloid with MOAB-2 in the subiculim of 1-, 2- and 4- month old 5xFAD mice. Scale bar 20 um (from PMID: 22423893). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: beta Amyloid Antibody (MOAB-2) - Immunofluorescent detection of beta Amyloid with MOAB-2 in the subiculim of 1-, 2- and 4- month old 5xFAD mice. Scale bar 20 um (from PMID: 22423893). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry: beta Amyloid Antibody (MOAB-2) - IHC of beta Amyloid on normal mouse brain (left) and 5xFAD mouse brain (right) using DAB with hematoxylin counterstain. The MOAB-2 antibody was used at 1:20 on normal mouse brain and at 1:400 on 5xFAD mouse brain. This image was taken for the unconjugated form of this product. Other forms have not been tested.