Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-77353 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal RBM12B Antibody
- Antibody type
- Polyclonal
- Description
- Affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 2 - 8 C 1 year from date of receipt
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RBM12B Antibody [NBP2-77353] - Samples: Whole cell lysate (10 ug) from Jurkat, HeLa, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RBM12B antibody NBP2-77353 used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RBM12B Antibody [NBP2-77353] - Detection of human RBM12B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RBM12B antibody NBP2-77353 used for IP at 6 ul per reaction. RBM12B was also immunoprecipitated by rabbit anti-RBM12B antibodyNBP2-77354. For blotting immunoprecipitated RBM12B, NBP2-77353 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.
