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- Antibody Data
- Antigen structure
- References [1]
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- Western blot [1]
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- Product number
- NBP1-77971 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-77971, RRID:AB_11003279
- Product name
- Mouse Monoclonal ESRP1/2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. This antibody reacts with both mouse Esrp-1 and Esrp-2 proteins.
- Reactivity
- Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition.
Warzecha CC, Jiang P, Amirikian K, Dittmar KA, Lu H, Shen S, Guo W, Xing Y, Carstens RP
The EMBO journal 2010 Oct 6;29(19):3286-300
The EMBO journal 2010 Oct 6;29(19):3286-300
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ESRP1/2 Antibody (23A7.C9) [NBP1-77971] - 293T cell extracts. Lane 1: GFP-transfected. Lane 2: Esrp-1 transfected (arrow). Lane 3: Esrp-2 transfected. Each lane contains approximately 5 ug of lysate. Primary antibody was used at a 1:1000 dilution in PBS-T plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse ECL antibody for 1hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.
