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- Antibody Data
- Antigen structure
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- Western blot [2]
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- Product number
- GTX48761 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX48761, RRID:AB_11162740
- Product name
- ESRP1 antibody [27H12]
- Antibody type
- Monoclonal
- Reactivity
- Mouse
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-ESRP-1 by western blot shows detection of ESRP-1 in transfected 293T cell extracts (lane 2, arrowhead). Lanes 1 and 3 contain GFP-transfected- and ESRP2-transfected 293T cell lysates, respectively. Briefly, each lane contains approximately 5 µg of lysate. Primary antibody was used at a 1:1000 dilution in PBS-T plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse ECL antibody for 1hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-ESRP-1 by western blot shows detection of ESRP-1 in transfected 293T cell extracts (lane 2, arrowhead). Lanes 1 and 3 contain GFP-transfected- and ESRP2-transfected 293T cell lysates, respectively. Briefly, each lane contains approximately 5 ?g ysate. Primary antibody was used at a 1:1000 dilution in PBST plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse secondary antibody for 1hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.
