Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF1714 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Contactin-2/TAG1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Contactin-2/TAG1 in direct ELISAs. Detects human, mouse, and rat Contactin-2/TAG1 in Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Contactin-3, rhContactin-4, rhContactin-5 and rhContactin-6 is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q02246
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Genetic Analysis of the Organization, Development, and Plasticity of Corneal Innervation in Mice.
Deletion of Nkx2-5 in trabecular myocardium reveals the developmental origins of pathological heterogeneity associated with ventricular non-compaction cardiomyopathy.
hPSC Modeling Reveals that Fate Selection of Cortical Deep Projection Neurons Occurs in the Subplate.
Lewis(x) and alpha2,3-sialyl glycans and their receptors TAG-1, Contactin, and L1 mediate CD24-dependent neurite outgrowth.
Bouheraoua N, Fouquet S, Marcos-Almaraz MT, Karagogeos D, Laroche L, Chédotal A
The Journal of neuroscience : the official journal of the Society for Neuroscience 2019 Feb 13;39(7):1150-1168
The Journal of neuroscience : the official journal of the Society for Neuroscience 2019 Feb 13;39(7):1150-1168
Deletion of Nkx2-5 in trabecular myocardium reveals the developmental origins of pathological heterogeneity associated with ventricular non-compaction cardiomyopathy.
Choquet C, Nguyen THM, Sicard P, Buttigieg E, Tran TT, Kober F, Varlet I, Sturny R, Costa MW, Harvey RP, Nguyen C, Rihet P, Richard S, Bernard M, Kelly RG, Lalevée N, Miquerol L
PLoS genetics 2018 Jul;14(7):e1007502
PLoS genetics 2018 Jul;14(7):e1007502
hPSC Modeling Reveals that Fate Selection of Cortical Deep Projection Neurons Occurs in the Subplate.
Ozair MZ, Kirst C, van den Berg BL, Ruzo A, Rito T, Brivanlou AH
Cell stem cell 2018 Jul 5;23(1):60-73.e6
Cell stem cell 2018 Jul 5;23(1):60-73.e6
Lewis(x) and alpha2,3-sialyl glycans and their receptors TAG-1, Contactin, and L1 mediate CD24-dependent neurite outgrowth.
Lieberoth A, Splittstoesser F, Katagihallimath N, Jakovcevski I, Loers G, Ranscht B, Karagogeos D, Schachner M, Kleene R
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 May 20;29(20):6677-90
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 May 20;29(20):6677-90
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Contactin-2/TAG1 by Western Blot. Western blot shows human cerebellum tissue lysate and cerebrospinal fluid (CSF). Recombinant Human (rh) Contactin-2/TAG1 (Catalog # 1714-CN) and conditioned media from BG01V human embryonic stem cells were loaded as positive and negative controls, respectively. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Contactin-2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1714) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Contactin-2/TAG1 at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Contactin-2/TAG1 in Human Brain. Contactin-2/TAG1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human Contactin-2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1714) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.