MA5-16671

antibody from Invitrogen Antibodies

Targeting: THY1 CD90

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-16671

Provider

Invitrogen Antibodies

Product name

CD90 Monoclonal Antibody (F15-42-1)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. A suggested positive control for immunohistochemical applications is human brain or thymus. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Antibody clone number

F15-42-1

Concentration

1 mg/mL

References

Submitted references

Quantification of Antibody Persistence for Cell Surface Protein Labeling.

Dempsey ME, Woodford-Berry O, Darling EM
Cellular and molecular bioengineering 2021 Jun;14(3):267-277

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GLI1 and AXIN2 Are Distinctive Markers of Human Calvarial Mesenchymal Stromal Cells in Nonsyndromic Craniosynostosis.

Di Pietro L, Barba M, Prampolini C, Ceccariglia S, Frassanito P, Vita A, Guadagni E, Bonvissuto D, Massimi L, Tamburrini G, Parolini O, Lattanzi W
International journal of molecular sciences 2020 Jun 19;21(12)

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Corneal keratocyte transition to mesenchymal stem cell phenotype and reversal using serum-free medium supplemented with fibroblast growth factor-2, transforming growth factor-β3 and retinoic acid.

Sidney LE, Hopkinson A
Journal of tissue engineering and regenerative medicine 2018 Jan;12(1):e203-e215

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Effect of culture medium on propagation and phenotype of corneal stroma-derived stem cells.

Sidney LE, Branch MJ, Dua HS, Hopkinson A
Cytotherapy 2015 Dec;17(12):1706-22

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of CD90 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD90 Monoclonal Antibody (F15-42-1) (Product # MA5-16671) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Green) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing membranous and cytosolic localization. Panel e represents MCF7 cells showing low expression of CD90. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HUT78 T monocytes using a CD90/Thy-1 monoclonal antibody (Product # MA5-16671)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HUT78 cells using a CD90/Thy-1 monoclonal antibody (Product # MA5-16671)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HUT78 T cells using a CD90/Thy-1 monoclonal antibody (Product # MA5-16671)