R32658

antibody from NSJ Bioreagents

Targeting: THY1 CD90

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

R32658

Provider

NSJ Bioreagents

Product name

CD90 Antibody

Antibody type

Polyclonal

Description

This highly specific CD90 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence applications with mouse and rat samples.

Reactivity

Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the CD90 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) mouse brain, 2) (m) thymus, 3) (m) lung, and 4) rat thymus lysate with CD90 antibody. Predicted molecular weight 18~35kDa depending on glycosylation level.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) mouse thymus, 2) mouse brain, 3) rat thymus and 4) rat brain tissue lysate with CD90 antibody. Predicted molecular weight 18~35 kDa depending on glycosylation level.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat brain, 2) rat thymus, 3) mouse brain and 4) mouse thymus tissue lysate with CD90 antibody. Predicted molecular weight 18~35 kDa depending on glycosylation level.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE rat brain tissue with CD90 antibody (green) and DAPI nuclear stain (blue). Required HIER: steam section in pH8 EDTA for 20 min and allow to cool prior to testing.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE mouse spleen tissue with CD90 antibody. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE mouse kidney tissue with CD90 antibody. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE mouse spleen tissue with CD90 antibody. Required HIER: steam section in pH8 EDTA for 20 min and allow to cool prior to testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE mouse brain tissue with CD90 antibody. Required HIER: steam section in pH8 EDTA for 20 min and allow to cool prior to testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE rat brain tissue with CD90 antibody. Required HIER: steam section in pH8 EDTA for 20 min and allow to cool prior to testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of mouse Neuro-2a cells with CD90 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD90 antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of mouse SP2/0 cells with CD90 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD90 antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of mouse YAC-1 cells with CD90 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD90 antibody.