Antibody data
- Antibody Data
- Antigen structure
- References [60]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [38]
Submit
Validation data
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- Product number
- 12-0909-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD90 (Thy-1) Monoclonal Antibody (eBio5E10 (5E10)), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBio5E10 monoclonal antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1). CD90 is a 25-35 kD receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, a small subset of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is expressed on a subset of immature, CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+. The CD90+CD34+ population is enriched for cells capable of long-term culture. CD90 is involved in regulation of adhesion and signal transduction by T cells. Applications Reported: This eBio5E10 (5E10) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio5E10 (5E10) antibody has been pre-titrated and tested by flow cytometric analysis of human erythroleukemia (HEL) cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- eBio5E10 (5E10)
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Fluorescence-activated cell sorting and phenotypic characterization of human fibro-adipogenic progenitors.
LIM Mineralization Protein-1 Enhances the Committed Differentiation of Dental Pulp Stem Cells through the ERK1/2 and p38 MAPK Pathways and BMP Signaling.
Tumor necrosis factor-α-primed mesenchymal stem cell-derived exosomes promote M2 macrophage polarization via Galectin-1 and modify intrauterine adhesion on a novel murine model.
Carboxymethyl chitin or chitosan for osteoinduction effect on the human periodontal ligament stem cells.
Exosomes derived from stem cells of human deciduous exfoliated teeth inhibit angiogenesis in vivo and in vitro via the transfer of miR-100-5p and miR-1246.
RNA-seq profiling of tubulointerstitial tissue reveals a potential therapeutic role of dual anti-phosphatase 1 in glomerulonephritis.
Single-cell Transcriptomic Analysis Reveals the Cellular Heterogeneity of Mesenchymal Stem Cells.
Mesenchymal stem cells transfer mitochondria to allogeneic Tregs in an HLA-dependent manner improving their immunosuppressive activity.
The Composition of Adipose-Derived Regenerative Cells Isolated from Lipoaspirate Using a Point of Care System Does Not Depend on the Subject's Individual Age, Sex, Body Mass Index and Ethnicity.
Fused Cells between Human-Adipose-Derived Mesenchymal Stem Cells and Monocytes Keep Stemness Properties and Acquire High Mobility.
Aberrant Expression of COX-2 and FOXG1 in Infrapatellar Fat Pad-Derived ASCs from Pre-Diabetic Donors.
Exosomes Regulate Interclonal Communication on Osteogenic Differentiation Among Heterogeneous Osteogenic Single-Cell Clones Through PINK1/Parkin-Mediated Mitophagy.
Low-Level Laser Irradiation Promotes Proliferation and Differentiation on Apical Papilla Stem Cells.
Wip1 regulates the immunomodulatory effects of murine mesenchymal stem cells in type 1 diabetes mellitus via targeting IFN-α/BST2.
Apoptotic vesicles restore liver macrophage homeostasis to counteract type 2 diabetes.
Therapeutic potential of human umbilical cord mesenchymal stem cells on aortic atherosclerotic plaque in a high-fat diet rabbit model.
Glycemic control by umbilical cord-derived mesenchymal stem cells promotes effects of fasting-mimicking diet on type 2 diabetic mice.
In Vitro Anti-cancer Activity of Adipose-Derived Mesenchymal Stem Cells Increased after Infection with Oncolytic Reovirus.
Mesenchymal stem cell exosome-derived miR-223 alleviates acute graft-versus-host disease via reducing the migration of donor T cells.
VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway.
Cell-mimicking nanodecoys neutralize SARS-CoV-2 and mitigate lung injury in a non-human primate model of COVID-19.
TRPA1 triggers hyperalgesia and inflammation after tooth bleaching.
Isolation and characterization of muscle stem cells, fibro-adipogenic progenitors, and macrophages from human skeletal muscle biopsies.
Down-Regulated Exosomal MicroRNA-221 - 3p Derived From Senescent Mesenchymal Stem Cells Impairs Heart Repair.
Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia.
Immunity-and-matrix-regulatory cells derived from human embryonic stem cells safely and effectively treat mouse lung injury and fibrosis.
The Effect of Angiotensin II, Retinoic Acid, EGCG, and Vitamin C on the Cardiomyogenic Differentiation Induction of Human Amniotic Fluid-Derived Mesenchymal Stem Cells.
Migration Inhibitory Factor in Conditioned Medium from Human Umbilical Cord Blood-Derived Mesenchymal Stromal Cells Stimulates Hair Growth.
PF-127 hydrogel plus sodium ascorbyl phosphate improves Wharton's jelly mesenchymal stem cell-mediated skin wound healing in mice.
TGF‑β induces periodontal ligament stem cell senescence through increase of ROS production.
Isolation and Culture of Human Stem Cells from Apical Papilla under Low Oxygen Concentration Highlight Original Properties.
Better therapeutic potential of bone marrow-derived mesenchymal stem cells compared with chorionic villi-derived mesenchymal stem cells in airway injury model.
LPS‑induced upregulation of the TLR4 signaling pathway inhibits osteogenic differentiation of human periodontal ligament stem cells under inflammatory conditions.
Bone mesenchymal stromal cells exhibit functional inhibition but no chromosomal aberrations in chronic myelogenous leukemia.
Visfatin Mediates Malignant Behaviors through Adipose-Derived Stem Cells Intermediary in Breast Cancer.
BNC1 regulates cell heterogeneity in human pluripotent stem cell-derived epicardium.
Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3β Complex in Hypophosphatasia.
CD90 promotes cell migration, viability and sphere‑forming ability of hepatocellular carcinoma cells.
Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta.
Human umbilical cord-derived mesenchymal stem cells ameliorate the enteropathy of food allergies in mice.
Spatial and Single-Cell Transcriptional Profiling Identifies Functionally Distinct Human Dermal Fibroblast Subpopulations.
Equine Dental Pulp Connective Tissue Particles Reduced Lameness in Horses in a Controlled Clinical Trial.
Effects of acute exposure to low-dose radiation on the characteristics of human bone marrow mesenchymal stromal/stem cells.
Nrf2 Inhibits Periodontal Ligament Stem Cell Apoptosis under Excessive Oxidative Stress.
Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism.
Endothelial and smooth muscle cells derived from human cardiac explants demonstrate angiogenic potential and suitable for design of cell-containing vascular grafts.
Distinct signaling programs control human hematopoietic stem cell survival and proliferation.
A Member of the Nuclear Receptor Superfamily, Designated as NR2F2, Supports the Self-Renewal Capacity and Pluripotency of Human Bone Marrow-Derived Mesenchymal Stem Cells.
CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa.
Human cytomegalovirus infection of human adipose-derived stromal/stem cells restricts differentiation along the adipogenic lineage.
Satellite cell response to erythropoietin treatment and endurance training in healthy young men.
Transendothelial migration of human umbilical mesenchymal stem cells across uterine endothelial monolayers: Junctional dynamics and putative mechanisms.
MIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia.
Vitamin D machinery and metabolism in porcine adipose-derived mesenchymal stem cells.
Human esophageal myofibroblasts secrete proinflammatory cytokines in response to acid and Toll-like receptor 4 ligands.
Impaired function of bone marrow stromal cells in systemic mastocytosis.
Immunophenotypic comparison of heterogenous non-sorted versus sorted mononuclear cells from human umbilical cord blood: a novel cell enrichment approach.
PDGFR-β (+) perivascular cells from infantile hemangioma display the features of mesenchymal stem cells and show stronger adipogenic potential in vitro and in vivo.
Epithelial cell differentiation of human mesenchymal stromal cells in decellularized lung scaffolds.
Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer.
Billeskov TB, Jensen JB, Jessen N, Farup J
STAR protocols 2023 Mar 17;4(1):102008
STAR protocols 2023 Mar 17;4(1):102008
LIM Mineralization Protein-1 Enhances the Committed Differentiation of Dental Pulp Stem Cells through the ERK1/2 and p38 MAPK Pathways and BMP Signaling.
Mu R, Chen B, Bi B, Yu H, Liu J, Li J, He M, Rong L, Liu B, Liu K, Zhu L, Shi X, Shuai Y, Jin L
International journal of medical sciences 2022;19(8):1307-1319
International journal of medical sciences 2022;19(8):1307-1319
Tumor necrosis factor-α-primed mesenchymal stem cell-derived exosomes promote M2 macrophage polarization via Galectin-1 and modify intrauterine adhesion on a novel murine model.
Li J, Pan Y, Yang J, Wang J, Jiang Q, Dou H, Hou Y
Frontiers in immunology 2022;13:945234
Frontiers in immunology 2022;13:945234
Carboxymethyl chitin or chitosan for osteoinduction effect on the human periodontal ligament stem cells.
Fan C, Li Z, Ji Q, Sun H, Liang Y, Yang P
Dental materials journal 2022 May 31;41(3):392-401
Dental materials journal 2022 May 31;41(3):392-401
Exosomes derived from stem cells of human deciduous exfoliated teeth inhibit angiogenesis in vivo and in vitro via the transfer of miR-100-5p and miR-1246.
Liu P, Zhang Q, Mi J, Wang S, Xu Q, Zhuang D, Chen W, Liu C, Zhang L, Guo J, Wu X
Stem cell research & therapy 2022 Mar 3;13(1):89
Stem cell research & therapy 2022 Mar 3;13(1):89
RNA-seq profiling of tubulointerstitial tissue reveals a potential therapeutic role of dual anti-phosphatase 1 in glomerulonephritis.
Park S, Lee H, Lee J, Lee S, Cho S, Huh H, Kim JY, Park M, Lee S, Kim Y, Choi M, Joo KW, Kim YS, Yang SH, Kim DK
Journal of cellular and molecular medicine 2022 Jun;26(12):3364-3377
Journal of cellular and molecular medicine 2022 Jun;26(12):3364-3377
Single-cell Transcriptomic Analysis Reveals the Cellular Heterogeneity of Mesenchymal Stem Cells.
Zhang C, Han X, Liu J, Chen L, Lei Y, Chen K, Si J, Wang TY, Zhou H, Zhao X, Zhang X, An Y, Li Y, Wang QF
Genomics, proteomics & bioinformatics 2022 Feb;20(1):70-86
Genomics, proteomics & bioinformatics 2022 Feb;20(1):70-86
Mesenchymal stem cells transfer mitochondria to allogeneic Tregs in an HLA-dependent manner improving their immunosuppressive activity.
Piekarska K, Urban-Wójciuk Z, Kurkowiak M, Pelikant-Małecka I, Schumacher A, Sakowska J, Spodnik JH, Arcimowicz Ł, Zielińska H, Tymoniuk B, Renkielska A, Siebert J, Słomińska E, Trzonkowski P, Hupp T, Marek-Trzonkowska NM
Nature communications 2022 Feb 14;13(1):856
Nature communications 2022 Feb 14;13(1):856
The Composition of Adipose-Derived Regenerative Cells Isolated from Lipoaspirate Using a Point of Care System Does Not Depend on the Subject's Individual Age, Sex, Body Mass Index and Ethnicity.
Schmitz C, Alt C, Azares AR, Pearce DA, Facile TR, Furia JP, Maffulli N, Huang C, Alt EU
Cells 2022 Dec 21;12(1)
Cells 2022 Dec 21;12(1)
Fused Cells between Human-Adipose-Derived Mesenchymal Stem Cells and Monocytes Keep Stemness Properties and Acquire High Mobility.
Montalbán-Hernández K, Casado-Sánchez C, Avendaño-Ortiz J, Casalvilla-Dueñas JC, Bonel-Pérez GC, Prado-Montero J, Valentín-Quiroga J, Lozano-Rodríguez R, Terrón-Arcos V, de la Bastida FR, Córdoba L, Laso-García F, Diekhorst L, Del Fresno C, López-Collazo E
International journal of molecular sciences 2022 Aug 26;23(17)
International journal of molecular sciences 2022 Aug 26;23(17)
Aberrant Expression of COX-2 and FOXG1 in Infrapatellar Fat Pad-Derived ASCs from Pre-Diabetic Donors.
O'Donnell BT, Monjure TA, Al-Ghadban S, Ives CJ, L'Ecuyer MP, Rhee C, Romero-Lopez M, Li Z, Goodman SB, Lin H, Tuan RS, Bunnell BA
Cells 2022 Aug 1;11(15)
Cells 2022 Aug 1;11(15)
Exosomes Regulate Interclonal Communication on Osteogenic Differentiation Among Heterogeneous Osteogenic Single-Cell Clones Through PINK1/Parkin-Mediated Mitophagy.
Fei D, Xia Y, Zhai Q, Wang Y, Zhou F, Zhao W, He X, Wang Q, Jin Y, Li B
Frontiers in cell and developmental biology 2021;9:687258
Frontiers in cell and developmental biology 2021;9:687258
Low-Level Laser Irradiation Promotes Proliferation and Differentiation on Apical Papilla Stem Cells.
Gutiérrez D, Rouabhia M, Ortiz J, Gaviria D, Alfonso C, Muñoz A, Inostroza C
Journal of lasers in medical sciences 2021;12:e75
Journal of lasers in medical sciences 2021;12:e75
Wip1 regulates the immunomodulatory effects of murine mesenchymal stem cells in type 1 diabetes mellitus via targeting IFN-α/BST2.
Zhou N, Liu W, Zhang W, Liu Y, Li X, Wang Y, Zheng R, Zhang Y
Cell death discovery 2021 Oct 29;7(1):326
Cell death discovery 2021 Oct 29;7(1):326
Apoptotic vesicles restore liver macrophage homeostasis to counteract type 2 diabetes.
Zheng C, Sui B, Zhang X, Hu J, Chen J, Liu J, Wu D, Ye Q, Xiang L, Qiu X, Liu S, Deng Z, Zhou J, Liu S, Shi S, Jin Y
Journal of extracellular vesicles 2021 May;10(7):e12109
Journal of extracellular vesicles 2021 May;10(7):e12109
Therapeutic potential of human umbilical cord mesenchymal stem cells on aortic atherosclerotic plaque in a high-fat diet rabbit model.
Li Y, Shi G, Han Y, Shang H, Li H, Liang W, Zhao W, Bai L, Qin C
Stem cell research & therapy 2021 Jul 15;12(1):407
Stem cell research & therapy 2021 Jul 15;12(1):407
Glycemic control by umbilical cord-derived mesenchymal stem cells promotes effects of fasting-mimicking diet on type 2 diabetic mice.
Zhao N, Gao YF, Bao L, Lei J, An HX, Pu FX, Cheng RP, Chen J, Ni H, Sui BD, Ji FP, Hu CH
Stem cell research & therapy 2021 Jul 13;12(1):395
Stem cell research & therapy 2021 Jul 13;12(1):395
In Vitro Anti-cancer Activity of Adipose-Derived Mesenchymal Stem Cells Increased after Infection with Oncolytic Reovirus.
Babaei A, Bannazadeh Baghi H, Nezhadi A, Jamalpoor Z
Advanced pharmaceutical bulletin 2021 Feb;11(2):361-370
Advanced pharmaceutical bulletin 2021 Feb;11(2):361-370
Mesenchymal stem cell exosome-derived miR-223 alleviates acute graft-versus-host disease via reducing the migration of donor T cells.
Liu W, Zhou N, Liu Y, Zhang W, Li X, Wang Y, Zheng R, Zhang Y
Stem cell research & therapy 2021 Feb 26;12(1):153
Stem cell research & therapy 2021 Feb 26;12(1):153
VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway.
Jiang W, Zhang J, Zhang X, Fan C, Huang J
Bioengineered 2021 Dec;12(2):10264-10284
Bioengineered 2021 Dec;12(2):10264-10284
Cell-mimicking nanodecoys neutralize SARS-CoV-2 and mitigate lung injury in a non-human primate model of COVID-19.
Li Z, Wang Z, Dinh PC, Zhu D, Popowski KD, Lutz H, Hu S, Lewis MG, Cook A, Andersen H, Greenhouse J, Pessaint L, Lobo LJ, Cheng K
Nature nanotechnology 2021 Aug;16(8):942-951
Nature nanotechnology 2021 Aug;16(8):942-951
TRPA1 triggers hyperalgesia and inflammation after tooth bleaching.
Chen C, Huang X, Zhu W, Ding C, Huang P, Li R
Scientific reports 2021 Aug 31;11(1):17418
Scientific reports 2021 Aug 31;11(1):17418
Isolation and characterization of muscle stem cells, fibro-adipogenic progenitors, and macrophages from human skeletal muscle biopsies.
Jensen JB, Møller AB, Just J, Mose M, de Paoli FV, Billeskov TB, Fred RG, Pers TH, Pedersen SB, Petersen KK, Bjerre M, Farup J, Jessen N
American journal of physiology. Cell physiology 2021 Aug 1;321(2):C257-C268
American journal of physiology. Cell physiology 2021 Aug 1;321(2):C257-C268
Down-Regulated Exosomal MicroRNA-221 - 3p Derived From Senescent Mesenchymal Stem Cells Impairs Heart Repair.
Sun L, Zhu W, Zhao P, Zhang J, Lu Y, Zhu Y, Zhao W, Liu Y, Chen Q, Zhang F
Frontiers in cell and developmental biology 2020;8:263
Frontiers in cell and developmental biology 2020;8:263
Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia.
Zhao AG, Shah K, Freitag J, Cromer B, Sumer H
Stem cells international 2020;2020:8898221
Stem cells international 2020;2020:8898221
Immunity-and-matrix-regulatory cells derived from human embryonic stem cells safely and effectively treat mouse lung injury and fibrosis.
Wu J, Song D, Li Z, Guo B, Xiao Y, Liu W, Liang L, Feng C, Gao T, Chen Y, Li Y, Wang Z, Wen J, Yang S, Liu P, Wang L, Wang Y, Peng L, Stacey GN, Hu Z, Feng G, Li W, Huo Y, Jin R, Shyh-Chang N, Zhou Q, Wang L, Hu B, Dai H, Hao J
Cell research 2020 Sep;30(9):794-809
Cell research 2020 Sep;30(9):794-809
The Effect of Angiotensin II, Retinoic Acid, EGCG, and Vitamin C on the Cardiomyogenic Differentiation Induction of Human Amniotic Fluid-Derived Mesenchymal Stem Cells.
Gasiūnienė M, Valatkaitė E, Navakauskaitė A, Navakauskienė R
International journal of molecular sciences 2020 Nov 19;21(22)
International journal of molecular sciences 2020 Nov 19;21(22)
Migration Inhibitory Factor in Conditioned Medium from Human Umbilical Cord Blood-Derived Mesenchymal Stromal Cells Stimulates Hair Growth.
Oh HA, Kwak J, Kim BJ, Jin HJ, Park WS, Choi SJ, Oh W, Um S
Cells 2020 May 28;9(6)
Cells 2020 May 28;9(6)
PF-127 hydrogel plus sodium ascorbyl phosphate improves Wharton's jelly mesenchymal stem cell-mediated skin wound healing in mice.
Deng Q, Huang S, Wen J, Jiao Y, Su X, Shi G, Huang J
Stem cell research & therapy 2020 Apr 3;11(1):143
Stem cell research & therapy 2020 Apr 3;11(1):143
TGF‑β induces periodontal ligament stem cell senescence through increase of ROS production.
Fan C, Ji Q, Zhang C, Xu S, Sun H, Li Z
Molecular medicine reports 2019 Oct;20(4):3123-3130
Molecular medicine reports 2019 Oct;20(4):3123-3130
Isolation and Culture of Human Stem Cells from Apical Papilla under Low Oxygen Concentration Highlight Original Properties.
Rémy M, Ferraro F, Le Salver P, Rey S, Genot E, Djavaheri-Mergny M, Thébaud N, Boiziau C, Boeuf H
Cells 2019 Nov 21;8(12)
Cells 2019 Nov 21;8(12)
Better therapeutic potential of bone marrow-derived mesenchymal stem cells compared with chorionic villi-derived mesenchymal stem cells in airway injury model.
Ji S, Wu C, Tong L, Wang L, Zhou J, Chen C, Song Y
Regenerative medicine 2019 Mar;14(3):165-177
Regenerative medicine 2019 Mar;14(3):165-177
LPS‑induced upregulation of the TLR4 signaling pathway inhibits osteogenic differentiation of human periodontal ligament stem cells under inflammatory conditions.
Yu B, Li Q, Zhou M
International journal of molecular medicine 2019 Jun;43(6):2341-2351
International journal of molecular medicine 2019 Jun;43(6):2341-2351
Bone mesenchymal stromal cells exhibit functional inhibition but no chromosomal aberrations in chronic myelogenous leukemia.
Xie J, Chen J, Wang B, He X, Huang H
Oncology letters 2019 Jan;17(1):999-1007
Oncology letters 2019 Jan;17(1):999-1007
Visfatin Mediates Malignant Behaviors through Adipose-Derived Stem Cells Intermediary in Breast Cancer.
Huang JY, Wang YY, Lo S, Tseng LM, Chen DR, Wu YC, Hou MF, Yuan SF
Cancers 2019 Dec 20;12(1)
Cancers 2019 Dec 20;12(1)
BNC1 regulates cell heterogeneity in human pluripotent stem cell-derived epicardium.
Gambardella L, McManus SA, Moignard V, Sebukhan D, Delaune A, Andrews S, Bernard WG, Morrison MA, Riley PR, Göttgens B, Gambardella Le Novère N, Sinha S
Development (Cambridge, England) 2019 Dec 13;146(24)
Development (Cambridge, England) 2019 Dec 13;146(24)
Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3β Complex in Hypophosphatasia.
Liu W, Zhang L, Xuan K, Hu C, Li L, Zhang Y, Jin F, Jin Y
Theranostics 2018;8(20):5575-5592
Theranostics 2018;8(20):5575-5592
CD90 promotes cell migration, viability and sphere‑forming ability of hepatocellular carcinoma cells.
Zhang K, Che S, Su Z, Zheng S, Zhang H, Yang S, Li W, Liu J
International journal of molecular medicine 2018 Feb;41(2):946-954
International journal of molecular medicine 2018 Feb;41(2):946-954
Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta.
Ventura Ferreira MS, Bienert M, Müller K, Rath B, Goecke T, Opländer C, Braunschweig T, Mela P, Brümmendorf TH, Beier F, Neuss S
Stem cell research & therapy 2018 Feb 5;9(1):28
Stem cell research & therapy 2018 Feb 5;9(1):28
Human umbilical cord-derived mesenchymal stem cells ameliorate the enteropathy of food allergies in mice.
Yan N, Xu J, Zhao C, Wu Y, Gao F, Li C, Zhou W, Xiao T, Zhou X, Shao Q, Xia S
Experimental and therapeutic medicine 2018 Dec;16(6):4445-4456
Experimental and therapeutic medicine 2018 Dec;16(6):4445-4456
Spatial and Single-Cell Transcriptional Profiling Identifies Functionally Distinct Human Dermal Fibroblast Subpopulations.
Philippeos C, Telerman SB, Oulès B, Pisco AO, Shaw TJ, Elgueta R, Lombardi G, Driskell RR, Soldin M, Lynch MD, Watt FM
The Journal of investigative dermatology 2018 Apr;138(4):811-825
The Journal of investigative dermatology 2018 Apr;138(4):811-825
Equine Dental Pulp Connective Tissue Particles Reduced Lameness in Horses in a Controlled Clinical Trial.
Bertone AL, Reisbig NA, Kilborne AH, Kaido M, Salmanzadeh N, Lovasz R, Sizemore JL, Scheuermann L, Kopp RJ, Zekas LJ, Brokken MT
Frontiers in veterinary science 2017;4:31
Frontiers in veterinary science 2017;4:31
Effects of acute exposure to low-dose radiation on the characteristics of human bone marrow mesenchymal stromal/stem cells.
Fujishiro A, Miura Y, Iwasa M, Fujii S, Sugino N, Andoh A, Hirai H, Maekawa T, Ichinohe T
Inflammation and regeneration 2017;37:19
Inflammation and regeneration 2017;37:19
Nrf2 Inhibits Periodontal Ligament Stem Cell Apoptosis under Excessive Oxidative Stress.
Liu Y, Yang H, Wen Y, Li B, Zhao Y, Xing J, Zhang M, Chen Y
International journal of molecular sciences 2017 May 17;18(5)
International journal of molecular sciences 2017 May 17;18(5)
Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism.
Li J, Mao Q, He J, She H, Zhang Z, Yin C
Stem cell research & therapy 2017 Mar 9;8(1):55
Stem cell research & therapy 2017 Mar 9;8(1):55
Endothelial and smooth muscle cells derived from human cardiac explants demonstrate angiogenic potential and suitable for design of cell-containing vascular grafts.
Zakharova IS, Zhiven' MK, Saaya SB, Shevchenko AI, Smirnova AM, Strunov A, Karpenko AA, Pokushalov EA, Ivanova LN, Makarevich PI, Parfyonova YV, Aboian E, Zakian SM
Journal of translational medicine 2017 Mar 3;15(1):54
Journal of translational medicine 2017 Mar 3;15(1):54
Distinct signaling programs control human hematopoietic stem cell survival and proliferation.
Knapp DJ, Hammond CA, Aghaeepour N, Miller PH, Pellacani D, Beer PA, Sachs K, Qiao W, Wang W, Humphries RK, Sauvageau G, Zandstra PW, Bendall SC, Nolan GP, Hansen C, Eaves CJ
Blood 2017 Jan 19;129(3):307-318
Blood 2017 Jan 19;129(3):307-318
A Member of the Nuclear Receptor Superfamily, Designated as NR2F2, Supports the Self-Renewal Capacity and Pluripotency of Human Bone Marrow-Derived Mesenchymal Stem Cells.
Zhu N, Wang H, Wang B, Wei J, Shan W, Feng J, Huang H
Stem cells international 2016;2016:5687589
Stem cells international 2016;2016:5687589
CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa.
Webber BR, Osborn MJ, McElroy AN, Twaroski K, Lonetree CL, DeFeo AP, Xia L, Eide C, Lees CJ, McElmurry RT, Riddle MJ, Kim CJ, Patel DD, Blazar BR, Tolar J
NPJ Regenerative medicine 2016;1:16014-
NPJ Regenerative medicine 2016;1:16014-
Human cytomegalovirus infection of human adipose-derived stromal/stem cells restricts differentiation along the adipogenic lineage.
Zwezdaryk KJ, Ferris MB, Strong AL, Morris CA, Bunnell BA, Dhurandhar NV, Gimble JM, Sullivan DE
Adipocyte 2016 Jan-Mar;5(1):53-64
Adipocyte 2016 Jan-Mar;5(1):53-64
Satellite cell response to erythropoietin treatment and endurance training in healthy young men.
Hoedt A, Christensen B, Nellemann B, Mikkelsen UR, Hansen M, Schjerling P, Farup J
The Journal of physiology 2016 Feb 1;594(3):727-43
The Journal of physiology 2016 Feb 1;594(3):727-43
Transendothelial migration of human umbilical mesenchymal stem cells across uterine endothelial monolayers: Junctional dynamics and putative mechanisms.
Ebrahim NA, Leach L
Placenta 2016 Dec;48:87-98
Placenta 2016 Dec;48:87-98
MIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia.
Yao Y, Deng Q, Song W, Zhang H, Li Y, Yang Y, Fan X, Liu M, Shang J, Sun C, Tang Y, Jin X, Liu H, Huang B, Zhou Y
Stem cell reports 2016 Aug 9;7(2):249-62
Stem cell reports 2016 Aug 9;7(2):249-62
Vitamin D machinery and metabolism in porcine adipose-derived mesenchymal stem cells.
Valle YL, Almalki SG, Agrawal DK
Stem cell research & therapy 2016 Aug 17;7(1):118
Stem cell research & therapy 2016 Aug 17;7(1):118
Human esophageal myofibroblasts secrete proinflammatory cytokines in response to acid and Toll-like receptor 4 ligands.
Gargus M, Niu C, Vallone JG, Binkley J, Rubin DC, Shaker A
American journal of physiology. Gastrointestinal and liver physiology 2015 Jun 1;308(11):G904-23
American journal of physiology. Gastrointestinal and liver physiology 2015 Jun 1;308(11):G904-23
Impaired function of bone marrow stromal cells in systemic mastocytosis.
Nemeth K, Wilson TM, Ren JJ, Sabatino M, Stroncek DM, Krepuska M, Bai Y, Robey PG, Metcalfe DD, Mezey E
Stem cell research 2015 Jul;15(1):42-53
Stem cell research 2015 Jul;15(1):42-53
Immunophenotypic comparison of heterogenous non-sorted versus sorted mononuclear cells from human umbilical cord blood: a novel cell enrichment approach.
Indumathi S, Harikrishnan R, Rajkumar JS, Dhanasekaran M
Cytotechnology 2015 Jan;67(1):107-14
Cytotechnology 2015 Jan;67(1):107-14
PDGFR-β (+) perivascular cells from infantile hemangioma display the features of mesenchymal stem cells and show stronger adipogenic potential in vitro and in vivo.
Yuan SM, Guo Y, Zhou XJ, Shen WM, Chen HN
International journal of clinical and experimental pathology 2014;7(6):2861-70
International journal of clinical and experimental pathology 2014;7(6):2861-70
Epithelial cell differentiation of human mesenchymal stromal cells in decellularized lung scaffolds.
Mendez JJ, Ghaedi M, Steinbacher D, Niklason LE
Tissue engineering. Part A 2014 Jun;20(11-12):1735-46
Tissue engineering. Part A 2014 Jun;20(11-12):1735-46
Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer.
Feng Y, Zhu M, Dangelmajer S, Lee YM, Wijesekera O, Castellanos CX, Denduluri A, Chaichana KL, Li Q, Zhang H, Levchenko A, Guerrero-Cazares H, Quiñones-Hinojosa A
Cell death & disease 2014 Dec 11;5(12):e1567
Cell death & disease 2014 Dec 11;5(12):e1567
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- Staining of human erythroleukemia (HEL) cells with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (blue histogram) or Anti-Human CD90 (Thy-1) PE (purple histogram). Total viable cells were used for analysis.
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- Fig. 1 Flow cytometry analysis of phenotype characterization of hUCMSCs. Phenotype of CD73, CD90, CD105, CD14, CD34, CD45, CD79a and HLA-DR of hUCMSCs was detected by flow cytometry. Intensity >= 95% represented strong expression while
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- Fig. 1 WJMSCs isolation and characterization. a Primary cell isolation procedure from Wharton''s jelly tissue. The migrated cells exhibited typical fibroblast-like morphology. Scale bar, 500 mum. b Flow cytometry analysis of P4 cells using mesenchymal stem cell markers (CD90, CD105, CD73), endothelial cell marker (CD31), and MHC class II protein HLA-DR. Isotypic antibodies (IgG1-PE and IgG1-FITC) were used as negative controls. c Representative stained images show that the fourth passage WJMSCs could differentiate into osteocytes (Alizarin Red S), adipocytes (Oil Red O), and chondrocytes (Alcian blue). Scale bar, 100 mum
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- FIGURE 1 Characterization of young and aged MSCs and exosomes. (A) Surface marker profiling of young-MSCs and aged-MSCs. (B) SA-beta-Gal staining showed that senescence increased significantly in aged MSCs. (C) Representative immunoblot images and quantitative analysis of p21, p53, and p16 protein level in young and aged-MSCs. ( n = 3). (D) Quantitation of cell cycle phases by propidium iodide staining. ( n = 3). (E) The CCK-8 assay showed that aged MSCs grew more slowly than young MSCs. ( n = 6). (F) Young and aged exosomes were observed using TEM. (G) The exosome surface markers were analyzed by Western blot. (H) Nanoparticle tracking analysis was used to analyze the particle size and concentration of Young-Exo and Aged-Exo. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; NS, not significant.
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- Fig. 8. BNC1 function in developing epicardial cells. (A) hPSC-epi developed from TET-inducible knockdown hPSC showed more than 90% reduction in BNC1 RNA under the TET condition (Aa) and 98% reduction at the protein level by western blot (Ab) as also visualised by immunofluorescence (Ac) ( n =5). (B,C) These cells showed more than 75% reduction of WT1 RNA (B) and a 5-fold increase in TCF21 RNA (C). (D,E) When BNC1 is silenced during its development, the hPSC-epi is enriched in the TCF21 high population as revealed by double immunofluorescence WT1/TCF21 (D) and THY1 flow cytometry analysis [E; histograms of a representative experiment (top) and recapitulative graph (bottom) of n =5; brackets indicate the percentage of positive cells]. (F) BNC1 silencing can be achieved in human foetal primary epicardium using siRNA as shown by RT-PCR (Fa) and immunofluorescence (Fb). The knockdown of BNC1 in human foetal primary epicardium leads to a greater than 5-fold increase in TCF21 RNA (Fc) ( n =3). The RT-PCR data shown in Aa, B, C, Fa and Fc were obtained by the quantitative relative standard curve protocol as described in Materials and Methods. RNA measurements were normalised to housekeeper genes porphobilinogen deaminase ( PBGD ) or GAPDH . Statistics were performed with Prism 7 from GraphPad with a ratio paired t -test. Error bars represent s.e.m. * P
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- Figure 1 Identification of dental pulp stem cells (DPSCs). Human DPSCs were positive for the cell surface antigens CD73, CD90, and CD105, as well as negative for CD14, CD20, CD34, and CD45 demonstrated by flow cytometry ( A ). DPSCs were cultured under osteogenic ( B , 14 days) or adipogenic ( C , 21 days) conditions, and showed mineralized nodules and lipid clusters as revealed by alizarin red and oil red staining, respectively. Scale bar = 400 ( B ) or 100 ( C ) mum.
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- Figure 2 Identification and purification of hPDLSCs by flow cytometry. hPDLSCs, human periodontal ligament stem cells.
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- Figure 1 Identification of ADSCs. ( A ) ADSCs were isolated from the adipose tissue of breast tumors. After two to three passages, the expressions of ADSCs markers (CD90FITC, CD105PE, and CD44FITC) and the lack of CD34PE and CD45FITC were confirmed by flow cytometry. ( B ) The differentiation ability of ADSCs was tested by adipogenesis, osteogenesis, and chondrogenesis.
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- Figure 1 AD-MSCs characterization; (A) Flow cytometry assay to assess the CD markers in the surface of extracted AD-MSCs. Target cells were positive for CD73 (95.1%), and CD90 (90.6%) surface markers and were negative for CD44 and CD34 markers. (B) Morphology of AD-MSCs at passage 3. (C) Oil red staining to prove the adipogenic potential differentiation of AD-MSCs. (D) Alizarin-red staining to confirm the osteogenic potential differentiation of AD-MSCs. Scale bar = 100 mum. Abbreviations: AD-MSCs: adipose-derived mesenchymal stem cells.
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- Fig. 1 Characterization of UCSCs. A UCSCs display a spindle shaped and fibroblast-like morphology. B High UCSCs expression of CD90, CD29, CD73, and CD105, and low expression of HLA using flow cytometry
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- Figure 1 Distribution of CD90 cells in human HCC cell lines. (A) Expression levels of CD90 in 4 different HCC cell lines (LM3, Huh7, MHCC 97L and SK-Hep-1) were determined by quantitative polymerase chain reaction. P
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- Figure 2 Effect of SK-Hep-1 CD90 + cells on cell cycle and viability. (A) Representative image and (B) quantitative analysis of flow cytometry assay to analyze the cell cycle of CD90 + and CD90 - cells. Data are presented as the mean +- standard deviation of three independent experiments. (C) MTT assay used to determine the viability of CD90 + and CD90 - cells. The results demonstrated that the viability of CD90 + cells was significantly increased compared with CD90 - cells. ** P
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- FIGURE 1 Single-cell colonies with high osteogenic ability had a greater mineralization promotion ability than L-SCCs. (A) The presence of cell surface markers of three SCCs was detected by flow cytometry. (B) The osteogenic and adipogenic differentiation potential of the three SCCs were assessed by Alizarin red and Oil Red O staining. (C) The osteogenic differentiation abilities of SCCs were tested by alkaline phosphatase (ALP) and Alizarin red staining. Alizarin red staining was quantified after the addition of 10% cetylpyridinium chloride. (D) Conditioned medium collected from H-SCCs or L-SCCs was mixed with equal volumes of twofold concentrated osteogenic induction medium, and was used to stimulate the TCs toward osteogenic differentiation for 7 days, following which, the levels of the osteogenic differentiation-related genes in the TCs were tested by RT-qPCR ( n = 3). (E) Conditioned medium collected from H-SCCs or L-SCCs was mixed with equal volumes of twofold concentrated osteogenic induction medium, and was used to stimulate the TCs toward osteogenic differentiation for 28 days, following which, the TCs were subjected to Alizarin red staining ( n = 3). CM, conditioned medium; TCs, target cells; AR, Alizarin red; N-CM, normal culture medium; L-CM, conditioned medium from L-SCCs; H-CM, conditioned medium from H-SCCs. Scale bar, 50 mum. * P < 0.05; ** P < 0.01; *** P < 0.001.
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- Figure 1. Characterizations of lung spheroid cell-derived nanodecoys. (a) Representative confocal images of LSCs labeled with ACE2, AQP5, and SFTPC antibodies. Three images were taken. Scale bars, 20 mum. (b) Representative flow cytometry analysis of LSCs (b) and EDCs (c) for ACE2 expression and (d) quantitative results of flow cytometry analysis of EDCs and LSCs for ACE2, EpCAM, CD90, MUC5b, and vWF. Data are shown as mean +- SD, n =4 or 6 independent experiments. Statistical analysis was performed by two-way ANOVA with a Tukey post hoc test. See Supplementary Figure S17 for gating strategies. (e) Size measurement of nanodecoys using NTA Nanosight. (f) Western blot of Alix and Calnexin in LSC-nanodecoys and LSCs. Flow cytometry analysis showing the expressions of ACE2 (g) and type II pneumocytes maker SFPTC (h) on LSC-nanodecoys. See Supplementary Figure S17 for gating strategies. (i) Measurement of ACE2 numbers on both cells and nanodecoys. HEK indicates HEK293. Data are shown as mean +- SD, n =3 independent experiments. Transmission electron microscopy (TEM) images showing naked nanodecoys (j) and enlarged figure (k). TEM images showing spike S1-bound nanodecoys (l) and enlarged figure (m). Spike S1 was detected using gold nanoparticle-labeled secondary antibodies with diameters of 10 nm. Cartoon pictures (insets in Figure j and l) were created with BioRender.com .
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- Figure 1. Morphology and immune phenotype of adipose-derived stem cells (ADSCs) were identified by morphological observation and flow cytometry. (a) Morphology of the primary (P1) and third passage (P3) of ADSCs. Images were acquired at 200x magnification. (b) Immune phenotype of ADSCs. The average data from three independent experiments were shown as mean +- standard deviation
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- Characterization and uptake of SHED-Exos. A Surface markers of SHED cells were analyzed by flow cytometry (FACS) and were positive for mesenchymal markers (CD44, CD105 and CD90) and negative for endothelial markers (CD45, CD19 and CD14). B The morphology of exosomes (indicated by arrows) was observed using a transmission electron microscope (TEM). Scale bar = 100 nm. C Particle size distribution of SHED-Exos assessed by nanoparticle tracking analysis (NTA). D Expression of exosome-specific CD63 and TSG101 validated using western blotting. E Efficient uptake of PKH67-labeled exosomes by HUVECs was detected at 24 h. Scale bars = 100 mum
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- Decreased expression of CD90, CD73, and CD105 on Pre-T2D IPFP-ASCs. ( A ) Flow cytometry for CD90 and CD105 demonstrated decreased expression in Pre-T2D IPFP-ASCs compared to those from Non-T2D and T2D groups ( n = 3, * p < 0.05). ( B ) CFU assay illustrated increased self-renewal properties in T2D IPFP-ASCs compared to Non-T2D ( n = 3, * p < 0.05). ( C - F ) RT-qPCR for common adipokines in ASCs and adipocyte differentiated ASCs demonstrated no significant difference in adipogenic potential between Non-T2D, Pre-T2D, and T2D IPFP-ASCs ( n = 3, * p < 0.05, *** p < 0.001, **** p < 0.0001). Non-T2D IPFP ASCs 7-Day is the control Group set as 1. Non-T2D: IPFP-ASCs from donors without Type II diabetes mellitus, Pre-T2D: IPFP-ASCs from donors with pre-Type II diabetes mellitus, T2D: IPFP-ASCs from donors with type II diabetes mellitus, 7D: Confluent ASCs, AQ: Adipocyte Differentiated ASCs.
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- Human-adipose-derived mesenchymal stem cells fuse with human monocytes ex vivo. ( A ), Schematic representation of experimental co-culture conditions. ( B ), Representative confocal images at 63x magnification of co-cultured DIO stained hADMSCs (green) with DID stained monocytes (red) and DAPI for nuclei (blue) is shown. Merges show co-cultured cells DID + DIO + (tangerine). ( C ), Representative confocal images at 63x magnification of DIO stained hADMSCs (green), DID stained monocytes (red) and hybrid cells (FHCs) (tangerine) are shown. DAPI was used for nuclei labelling (blue). Diameter sizes are shown in merge panels. ( D ) , Diameter comparisons between hADMSCs (green), monocytes (red), and FHCs (tangerine) are shown (n = 3). ( E ), Left panels, representative FACS analysis gating strategy illustrating CD90 + and CD14 + single staining's. Right panel, dot plot after 5-day co-culture illustrates CD90 + hADMSCs (green), CD14 + human monocytes (red), and CD90 + CD14 + (FHCs, tangerine). **, p < 0.01; ***, p < 0.001; ****, p < 0.0001 in one-way ANOVA test with Tukey's multiple comparison post-hoc test.
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- Figure 1 Primary human adipose-derived cells cultured in hypoxia (hAMSCs-H) and normoxia (hAMSCs-N) are both MSCs but normoxia-cultured cells show increased signs of senescence, such as increased area and elongated morphology, compared with hypoxia-cultured cells. ( a ) hAMSCs were isolated from human fat tissue and cultured in hypoxic (1.5% oxygen) or normoxic (21% oxygen) conditions in vitro . The viability, mobility, tumor tropism, safety, and tumorigenic potential were subsequently compared in vitro and in vivo . ( b ) Differentiation assay. hAMSCs were cultured in control media and differentiation media for 3 weeks, 10 days after the second passage. Three different stains were performed to assess differentiation capabilities (scale bar, 100 mu m). ( c ) Flow cytometric analysis was performed to confirm the absence of CD31-, CD34-, and CD45-positive cells in both cell cultures. In addition, primary hAMSC cultures expressed high levels of CD73, CD90, and CD105, both in hypoxic and normoxic culture conditions at day 10 after passage 2. ( d ) Representative images of cell morphologies of hAMSCs on 2D surface (scale bar, 200 mu m). ( e ) Schematic of 3D-nanopatterned surface used to assess morphology and motility. ( f ) Images of cell morphologies of hAMSCs on 3D-nanopatterned surface (scale bar, 200 mu m). ( g - j ) The length, width, area, and length-to-width ratio were measured and compared after cell aligned on the nanopattern surface. Error bars represent S.E.M. * P
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- Figure 3 Hypoxia-cultured primary human adipose-derived mesenchymal stem cells (hAMSCs-H) retain a greater proliferation capacity compared with normoxia-cultured primary hAMSCs (hAMSCs-N) when exposed to GBM media. hAMSCs-H maintain stem cell characteristics when exposed to GBM media. ( a ) Representative MRI of GBM from a patient. ( b ) Schema showing the collection of GBM CM and culture of hAMSCs in filtered GBM CM for proliferation and migration assays. ( c ) MTT assay was used to determine the effects of hypoxic conditions on the proliferative capacity of primary hAMSCs in GBM CM. In GBM CM, hAMSCs-H showed greater proliferation at day 10 and 15 compared with hAMSCs-N. ( d ) Ki-67 immunostaining was performed to quantify the number of proliferating cells in GBM CM. Proliferative capacities of hAMSCs-H and hAMSCs-N are shown in GBM CM (normalized to hAMSC-N proliferative capacity in control media). In GBM CM, hAMSCs-H had a greater proportion of proliferating cells than hAMSCs-N. ( e) Differentiation assay. hAMSCs were cultured in control media, differentiation media, and GBM CM for 3 weeks, 10 days after the second passage. Three stainings were performed to assess the differentiation capabilities (scale bar, 100 mu m). Both hAMSCs-N and hAMSCs-H maintained tri-lineage differentiation capability in GBM CM. ( f ) Flow cytometric analysis for CD31, CD34, CD45, CD73, CD90, and CD105 in hAMSC-N and hAMSC-H cultures after exposure to GBM CM for 20 days. hAMSCs-H maintained MSC
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- Figure 1 Characteristics of BM-MSCs. (a) Representative morphology of BM-MSCs. Scale bar = 500 mu m. (b) Representative flow cytometric characterization of cell surface markers expressed on BM-MSCs. Isotypic controls were represented by the gray filled histograms.
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- Figure 2 Characteristics of transfected BM-MSCs. (a) Representative morphology of transfected BM-MSCs in negative control group. (b) More than 90% of BM-MSCs expressed GFP in negative control group. (c) Representative morphology of transfected BM-MSCs in knock-down group. (d) More than 90% of BM-MSCs expressed GFP in knock-down group. (e) Representative flow cytometric characterization of cell surface markers expressed on transfected BM-MSCs. Isotypic controls were represented by black line. The red line represented the negative control group and the blue line represented the knock-down group. (f) The knock-down of NR2F2 was confirmed by western blot analysis.
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- Figure 2 CESCs Shared Features with BM-MSCs Regarding Morphology, Stem Cell Surface Markers, and Differentiation Ability (A) H&E staining of the tissue section. (B) Morphology of CESCs in agarose after seeding 6 weeks later. (C) Histologic section stained with Alcian blue of chondrified pellets in which CESCs formed in chondrogenic induction medium after 3 weeks. (D) Alizarin red staining of CESCs that underwent osteogenic induction for 3 weeks. (E) Immunophenotypic profile of stem cells in CESCs by flow cytometric analysis. The green lines indicate the fluorescence intensity of cells stained with the corresponding antibodies, and the red lines represent isotype-matched negative control cells. (F) Percentages of CESCs expressing different stem cell markers (n = 6 independent experiments). Data represent the mean +- SD.
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- Figure 3 Mesenchymal stem cell derivation. ( a ) MSC differentiation. Mono-layer iPSCs were subjected to bFGF, PDGFab and EGF resulting in differentiation to a cell population with spindle-shaped morphology (right). ( b ) FACS analysis. Passage 3 MSCs were analysed for cell surface expression of CD73, CD105, and CD90 ( n =3 experiments), and histogram analysis is shown in blue. Isotype antibody control FACS histograms are shown in pink. ( c , d ) Tri-lineage differentiation. ( c ) Oil red-O staining demonstrating the ability of iPSC-derived MSCs to form adipose cells. ( d ) Alizarin red staining of osteogenic progeny. ( e ) Toluidine blue staining of chondrogenic cells from MSCs. ( c - e ) Representative images of at least two different MSC pools and n =3-4 replicates. FACS, fluorescence-activated cell sorting.
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- Figure 3 Mesenchymal stem cell derivation. ( a ) MSC differentiation. Mono-layer iPSCs were subjected to bFGF, PDGFab and EGF resulting in differentiation to a cell population with spindle-shaped morphology (right). ( b ) FACS analysis. Passage 3 MSCs were analysed for cell surface expression of CD73, CD105, and CD90 ( n =3 experiments), and histogram analysis is shown in blue. Isotype antibody control FACS histograms are shown in pink. ( c , d ) Tri-lineage differentiation. ( c ) Oil red-O staining demonstrating the ability of iPSC-derived MSCs to form adipose cells. ( d ) Alizarin red staining of osteogenic progeny. ( e ) Toluidine blue staining of chondrogenic cells from MSCs. ( c - e ) Representative images of at least two different MSC pools and n =3-4 replicates. FACS, fluorescence-activated cell sorting.
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- Fig. 1 Cells isolated from cardiac explants in culture. a Morphology of primary cells migrated from cardiac explants. Phase contrast images of cells cultivated in an endothelial growth medium ( left panel ) and cells derived in a smooth muscle growth medium ( right panel ). Scale bar 100 mum. b CD31-positive endothelial ( left panel ) and alphaSMA-positive smooth muscle ( right panel ) cells were detected by immunofluorescent staining of primary cardiac explant cultures growing in endothelial or smooth muscle medium, respectively. Scale bar 100 mum. c Flow cytometric analysis of surface markers. Comparison of cells cultivated in endothelial growth medium before and after MACS separation ( left panel ). Comparison of cells cultivated in smooth muscle cell growth medium at the second and fifth passages ( right panel )
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- Fig. 2 a Cumulative population-doubling (cPD) levels versus passage number for the four different sources of MSC. Black represents CV-MSC ( n = 7), dark gray UC-MSC ( n = 4), medium gray AT-MSC ( n = 5), and light gray BM-MSC ( n = 6). b IHC-based senescence-associated beta-galactosidase (SA-beta-gal) staining of CV-MSC in early (i, passage 4) and late (ii, passage 9) passages, AT-MSC in passage 6 (iii), BM-MSC in passage 6 (iv), and UC-MSC in passage 2 (v) and passage 4 (vi). Scale = 200 mum. c IHC of CV-MSC (i, ii) and BM-MSC (iii, iv) stained for osteopontin (i, iii) and fibronectin (ii, iv). Scale = 1 mm. d Collagen area (%) after collagen contraction assay for CV-MSC ( n = 4), BM-MSC ( n = 3), UC-MSC ( n = 4), and AT-MSC ( n = 3). Cells in passage 3 were used. Results expressed as mean +- SD, percentage of the total collagen area of the collagen gels without cells. e Surface marker expression of CV-MSC in early passages ( n = 5). Results expressed as mean +- SD (%). f Representative immunofluorescence of early passaged CV-MSC (i, iii) and BM-MSC (iii, iv) stained for SM22alpha (i, iii) and alpha-SMA (ii, iv). Scale = 50 mum. AT adipose tissue, BM bone marrow, CV chorionic villi, MSC mesenchymal stromal cells, UC umbilical cord
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- Figure 1. Characterization of PDLSCs. (A) PDL cell clusters exhibited radiating or whirlpool-like morphology. The central structure in this image is a fragment of PDL tissue. Scale bar, 200 mum (B) CD146 + PDLSCs were small, round, fusiform and triangular. Scale bar, 100 mum. (C) PDLSCs were positive for the stem cell markers CD44, CD90 and CD105, but negative for CD34 and CD45, as detected by flow cytometry. PDL, periodontal ligament; PDLSCs, PDL stem cells.
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- Figure 1. Characterization of hUC-MSCs. (A) Morphological observations of hUC-MSCs. Umbilical cord tissues were cultured for >15 days and long spindle-shaded fibroblastic cells were observed around the tissue using Zeiss light microscopy (scale bar, 100 um). (B) Phenotyping of hUC-MSCs. hUC-MSCs were stained with a fluorescein-labeled antibodies (CD34, CD45, CD73, CD90, CD105, CD14, CD19 and HLA-DR) and analyzed with a flow cytometer. (C) Adipogenic and (D) osteogenic differentiation of hUC-MSCs. hUC-MSCs were cultured in adipogenic and osteogenic medium, respectively. Lipid droplets in the adipocytes are presented with Oil Red O staining (scale bar, 100 um). hUC-MSCs-derived osteoblasts were detected with Alizarin Red staining (scale bar, 200 um). (E) hUC-MSCs inhibit the proliferation of CFSE-labeled CD4 + T cells, which were activated by Con A stimulation. Experiments were repeated three times and representative graphs and images are presented. hUC-MSC, human umbilical cord-derived mesenchymal stem cell; MSC Sup, culture supernatant of hUC-MSCs; Con A, concanavalin A; CFSE, carboxyfluorescein succinimidyl ester.
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- Figure 4 Protein expression analysis. SCAPs from EXP III (early passages of UBx-SCAP) were grown at 3% or 21% O 2 . ( A ) Graphs showing flow cytometry analysis of different markers as indicated ((% of positive cells, left column) and mean of fluorescence intensity (MFI, in arbitrary units, right column)). Numbers 1, 2, 3 refer to the three individuals. For each group, three to six samples from different early passages were analyzed. Statistical analyses were done with a Mann Whitney test. * p < 0.05. ( B ) Western blot analysis of UBx-SCAP-1 (early passage) grown under 21% or 3% O 2 and of human iPSCs (induced pluripotent stem cells, IMR90 cell line) used as a positive control for expression of Oct4 and Nanog. ERK2 was used as the loading control.
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- Figure 2 Flow cytometry of CD cell surface markers for cells cultured under hypoxia and normoxia. The positive CD markers for MSCs as detected by the fluorescent antibodies anti-CD73 FITC, anti-CD105 PE, and anti-CD90 PE Cy7. The negative markers of MSCs were detected using anti-CD14 FITC, anti-CD45 PerCP, anti-CD34-R-PE, and anti-CD19 PE-Cy7 antibodies. Unstained cell for each condition was used as negative controls.
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- Figure 1 Human amniotic fluid-derived mesenchymal stem cells (AF-MSCs) characterization. ( A ) The typical morphology of human amniotic fluid-derived mesenchymal stem cells, grown in cell culture. Scale bar = 400 um. ( B ) The expression of the main cell surface markers CD44, CD90, CD105, and CD34 as detected by flow cytometry. Unlabeled ctrl: unlabeled, undifferentiated control cells. Results are presented as the mean +- SD ( n = 3). ( C ) The relative expression of pluripotency gene markers, namely, OCT4, SOX2, NANOG, and REX1, as determined by RT-qPCR. Data, relative to GAPDH, are presented as the mean +- SD ( n = 3).
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