Antibody data
- Antibody Data
- Antigen structure
- References [20]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 14-0909-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD90 (Thy-1) Monoclonal Antibody (eBio5E10 (5E10)), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBio5E10 monoclonal antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1). CD90 is a 25-35 kD receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, a small subset of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is expressed on a subset of immature, CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+. The CD90+CD34+ population is enriched for cells capable of long-term culture. CD90 is involved in regulation of adhesion and signal transduction by T cells. Applications Reported: This eBio5E10 (5E10) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistology staining of frozen tissue sections. Applications Tested: This eBio5E10 (5E10) antibody has been tested by flow cytometric analysis of human erythroleukemia (HEL) cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- eBio5E10 (5E10)
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references TGF‑β induces periodontal ligament stem cell senescence through increase of ROS production.
BNC1 regulates cell heterogeneity in human pluripotent stem cell-derived epicardium.
Thioredoxin 1 is associated with the proliferation and apoptosis of rheumatoid arthritis fibroblast-like synoviocytes.
Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta.
Spatial and Single-Cell Transcriptional Profiling Identifies Functionally Distinct Human Dermal Fibroblast Subpopulations.
A mobile endocytic network connects clathrin-independent receptor endocytosis to recycling and promotes T cell activation.
Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism.
Endothelial and smooth muscle cells derived from human cardiac explants demonstrate angiogenic potential and suitable for design of cell-containing vascular grafts.
A Member of the Nuclear Receptor Superfamily, Designated as NR2F2, Supports the Self-Renewal Capacity and Pluripotency of Human Bone Marrow-Derived Mesenchymal Stem Cells.
CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa.
MIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia.
Pericyte response to contraction mode-specific resistance exercise training in human skeletal muscle.
Role of intestinal myofibroblasts in HIV-associated intestinal collagen deposition and immune reconstitution following combination antiretroviral therapy.
Human esophageal myofibroblasts secrete proinflammatory cytokines in response to acid and Toll-like receptor 4 ligands.
Immunophenotypic comparison of heterogenous non-sorted versus sorted mononuclear cells from human umbilical cord blood: a novel cell enrichment approach.
Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells.
PDGFR-β (+) perivascular cells from infantile hemangioma display the features of mesenchymal stem cells and show stronger adipogenic potential in vitro and in vivo.
Epithelial cell differentiation of human mesenchymal stromal cells in decellularized lung scaffolds.
Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer.
Expression of Thy-1 on human hematopoietic progenitor cells.
Fan C, Ji Q, Zhang C, Xu S, Sun H, Li Z
Molecular medicine reports 2019 Oct;20(4):3123-3130
Molecular medicine reports 2019 Oct;20(4):3123-3130
BNC1 regulates cell heterogeneity in human pluripotent stem cell-derived epicardium.
Gambardella L, McManus SA, Moignard V, Sebukhan D, Delaune A, Andrews S, Bernard WG, Morrison MA, Riley PR, Göttgens B, Gambardella Le Novère N, Sinha S
Development (Cambridge, England) 2019 Dec 13;146(24)
Development (Cambridge, England) 2019 Dec 13;146(24)
Thioredoxin 1 is associated with the proliferation and apoptosis of rheumatoid arthritis fibroblast-like synoviocytes.
Lu T, Zong M, Fan S, Lu Y, Yu S, Fan L
Clinical rheumatology 2018 Jan;37(1):117-125
Clinical rheumatology 2018 Jan;37(1):117-125
Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta.
Ventura Ferreira MS, Bienert M, Müller K, Rath B, Goecke T, Opländer C, Braunschweig T, Mela P, Brümmendorf TH, Beier F, Neuss S
Stem cell research & therapy 2018 Feb 5;9(1):28
Stem cell research & therapy 2018 Feb 5;9(1):28
Spatial and Single-Cell Transcriptional Profiling Identifies Functionally Distinct Human Dermal Fibroblast Subpopulations.
Philippeos C, Telerman SB, Oulès B, Pisco AO, Shaw TJ, Elgueta R, Lombardi G, Driskell RR, Soldin M, Lynch MD, Watt FM
The Journal of investigative dermatology 2018 Apr;138(4):811-825
The Journal of investigative dermatology 2018 Apr;138(4):811-825
A mobile endocytic network connects clathrin-independent receptor endocytosis to recycling and promotes T cell activation.
Compeer EB, Kraus F, Ecker M, Redpath G, Amiezer M, Rother N, Nicovich PR, Kapoor-Kaushik N, Deng Q, Samson GPB, Yang Z, Lou J, Carnell M, Vartoukian H, Gaus K, Rossy J
Nature communications 2018 Apr 23;9(1):1597
Nature communications 2018 Apr 23;9(1):1597
Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism.
Li J, Mao Q, He J, She H, Zhang Z, Yin C
Stem cell research & therapy 2017 Mar 9;8(1):55
Stem cell research & therapy 2017 Mar 9;8(1):55
Endothelial and smooth muscle cells derived from human cardiac explants demonstrate angiogenic potential and suitable for design of cell-containing vascular grafts.
Zakharova IS, Zhiven' MK, Saaya SB, Shevchenko AI, Smirnova AM, Strunov A, Karpenko AA, Pokushalov EA, Ivanova LN, Makarevich PI, Parfyonova YV, Aboian E, Zakian SM
Journal of translational medicine 2017 Mar 3;15(1):54
Journal of translational medicine 2017 Mar 3;15(1):54
A Member of the Nuclear Receptor Superfamily, Designated as NR2F2, Supports the Self-Renewal Capacity and Pluripotency of Human Bone Marrow-Derived Mesenchymal Stem Cells.
Zhu N, Wang H, Wang B, Wei J, Shan W, Feng J, Huang H
Stem cells international 2016;2016:5687589
Stem cells international 2016;2016:5687589
CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa.
Webber BR, Osborn MJ, McElroy AN, Twaroski K, Lonetree CL, DeFeo AP, Xia L, Eide C, Lees CJ, McElmurry RT, Riddle MJ, Kim CJ, Patel DD, Blazar BR, Tolar J
NPJ Regenerative medicine 2016;1
NPJ Regenerative medicine 2016;1
MIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia.
Yao Y, Deng Q, Song W, Zhang H, Li Y, Yang Y, Fan X, Liu M, Shang J, Sun C, Tang Y, Jin X, Liu H, Huang B, Zhou Y
Stem cell reports 2016 Aug 9;7(2):249-62
Stem cell reports 2016 Aug 9;7(2):249-62
Pericyte response to contraction mode-specific resistance exercise training in human skeletal muscle.
Farup J, De Lisio M, Rahbek SK, Bjerre J, Vendelbo MH, Boppart MD, Vissing K
Journal of applied physiology (Bethesda, Md. : 1985) 2015 Nov 15;119(10):1053-63
Journal of applied physiology (Bethesda, Md. : 1985) 2015 Nov 15;119(10):1053-63
Role of intestinal myofibroblasts in HIV-associated intestinal collagen deposition and immune reconstitution following combination antiretroviral therapy.
Asmuth DM, Pinchuk IV, Wu J, Vargas G, Chen X, Mann S, Albanese A, Ma ZM, Saroufeem R, Melcher GP, Troia-Cancio P, Torok NJ, Miller CJ, Powell DW
AIDS (London, England) 2015 May 15;29(8):877-88
AIDS (London, England) 2015 May 15;29(8):877-88
Human esophageal myofibroblasts secrete proinflammatory cytokines in response to acid and Toll-like receptor 4 ligands.
Gargus M, Niu C, Vallone JG, Binkley J, Rubin DC, Shaker A
American journal of physiology. Gastrointestinal and liver physiology 2015 Jun 1;308(11):G904-23
American journal of physiology. Gastrointestinal and liver physiology 2015 Jun 1;308(11):G904-23
Immunophenotypic comparison of heterogenous non-sorted versus sorted mononuclear cells from human umbilical cord blood: a novel cell enrichment approach.
Indumathi S, Harikrishnan R, Rajkumar JS, Dhanasekaran M
Cytotechnology 2015 Jan;67(1):107-14
Cytotechnology 2015 Jan;67(1):107-14
Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells.
Byrne SM, Ortiz L, Mali P, Aach J, Church GM
Nucleic acids research 2015 Feb 18;43(3):e21
Nucleic acids research 2015 Feb 18;43(3):e21
PDGFR-β (+) perivascular cells from infantile hemangioma display the features of mesenchymal stem cells and show stronger adipogenic potential in vitro and in vivo.
Yuan SM, Guo Y, Zhou XJ, Shen WM, Chen HN
International journal of clinical and experimental pathology 2014;7(6):2861-70
International journal of clinical and experimental pathology 2014;7(6):2861-70
Epithelial cell differentiation of human mesenchymal stromal cells in decellularized lung scaffolds.
Mendez JJ, Ghaedi M, Steinbacher D, Niklason LE
Tissue engineering. Part A 2014 Jun;20(11-12):1735-46
Tissue engineering. Part A 2014 Jun;20(11-12):1735-46
Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer.
Feng Y, Zhu M, Dangelmajer S, Lee YM, Wijesekera O, Castellanos CX, Denduluri A, Chaichana KL, Li Q, Zhang H, Levchenko A, Guerrero-Cazares H, Quiñones-Hinojosa A
Cell death & disease 2014 Dec 11;5:e1567
Cell death & disease 2014 Dec 11;5:e1567
Expression of Thy-1 on human hematopoietic progenitor cells.
Craig W, Kay R, Cutler RL, Lansdorp PM
The Journal of experimental medicine 1993 May 1;177(5):1331-42
The Journal of experimental medicine 1993 May 1;177(5):1331-42
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of human erythroleukemia (HEL) cell line with 0.25 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.25 µg of Anti-Human CD90 (Thy-1) Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Total viable cells were used for analysis.