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Learn14073-1-AP
antibody from Invitrogen Antibodies
Targeting: SRPK1
SFRSK1
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 14073-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SRPK1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: QEESESPVE RPLKENPPNK MTQEKLEESS TIGQDQTLME RDTEGGAAEI NCNGVIEVIN YTQNSNNETL RHKEDLHNAN DCDVQNLNQE SSFLSSQNGD SSTSQETDSC TPITSEVSDT MVCQSSSTVG QSFSEQHISQ LQESIRAEIP CEDEQEQEHN GPLDNKGKST AGNFLVNPLE PKNAEKLKVK IADLGNACWV HKHFTEDIQT RQYRSLEVLI GSGYNTPADI WSTACMAFEL ATGDYLFEPH SGEEYTRDED HIALIIELLG KVPRKLIVAG KYSKEFFTKK GDLKHITKLK PWGLFEVLVE KYEWSQEEAA GFTDFLLPML ELIPEKRATA AECLRHPWLN S (306-655 aa encoded by BC038292)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.27 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- L02 cells were subjected to SDS PAGE followed by western blot with 14073-1-AP (SRPK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- L02 cells were subjected to SDS PAGE followed by western blot with 14073-1-AP (SRPK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 14073-1-AP (SRPK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SH-SY5Y cells using 14073-1-AP (SRPK1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14073-1-AP (SRPK1 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14073-1-AP (SRPK1 Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 14073-1-AP (SRPK1 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 14073-1-AP (SRPK1 Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14073-1-AP ( SRPK1 Antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14073-1-AP ( SRPK1 Antibody) at dilution of 1:200 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 MCF-7 cells were stained with 0.2ug SRPK1 antibody (14073-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-SRPK1 (IP:14073-1-AP, 3ug; Detection:14073-1-AP 1:1000) with mouse testis tissue lysate 3000ug.
