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- Immunohistochemistry [1]
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- Product number
- PA5-103499 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GOLT1B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of total GOLT1B.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Multi-Omics Analyses Revealed GOLT1B as a Potential Prognostic Gene in Breast Cancer Probably Regulating the Immune Microenvironment.
Liu J, Zhang W, Cai W, Chen Y, Cai X, Tang D, Tang M, Dai Y
Frontiers in oncology 2021;11:805273
Frontiers in oncology 2021;11:805273
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GOLT1B in rat heart tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with GOLT1B polyclonal antibody (Product # PA5-103499) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 The mRNA and protein expression of GOLT1B was up-regulated in human breast cancer. The expression of GOLT1B in breast cancer tissues versus normal tissues was investigated using the datasets from (A) the GEPIA database, (B) the UALCAN database, and (C) the TCGA database. (D) The GOLT1B expression in 112 paired breast cancer tissues and adjacent normal tissues using the datasets from TCGA database. (E) The protein expression of GOLT1B in breast cancer tissues and normal breast tissues was evaluated through immunohistochemical tests from The Human Protein Atlas database. (F) Western blotting detecting GOLT1B expression in normal mammary epithelial cell line and breast cancer cell lines. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
