Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF5647 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal FoxP2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and mouse FoxP2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) FoxP1, rhFoxP3, and rhFoxP4 is observed.
- Reactivity
- Human, Mouse
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Chemosensory modulation of neural circuits for sodium appetite.
Efferent projections of excitatory and inhibitory preBötzinger Complex neurons.
Expression of the transcription factor FOXP2 in brainstem respiratory circuits of adult rat is restricted to upper-airway pre-motor areas.
Kölliker-Fuse GABAergic and glutamatergic neurons project to distinct targets.
Barrington's nucleus: Neuroanatomic landscape of the mouse "pontine micturition center".
HSD2 neurons in the hindbrain drive sodium appetite.
Lee S, Augustine V, Zhao Y, Ebisu H, Ho B, Kong D, Oka Y
Nature 2019 Apr;568(7750):93-97
Nature 2019 Apr;568(7750):93-97
Efferent projections of excitatory and inhibitory preBötzinger Complex neurons.
Yang CF, Feldman JL
The Journal of comparative neurology 2018 Jun 1;526(8):1389-1402
The Journal of comparative neurology 2018 Jun 1;526(8):1389-1402
Expression of the transcription factor FOXP2 in brainstem respiratory circuits of adult rat is restricted to upper-airway pre-motor areas.
Stanić D, Dhingra RR, Dutschmann M
Respiratory physiology & neurobiology 2018 Apr;250:14-18
Respiratory physiology & neurobiology 2018 Apr;250:14-18
Kölliker-Fuse GABAergic and glutamatergic neurons project to distinct targets.
Geerling JC, Yokota S, Rukhadze I, Roe D, Chamberlin NL
The Journal of comparative neurology 2017 Jun 1;525(8):1844-1860
The Journal of comparative neurology 2017 Jun 1;525(8):1844-1860
Barrington's nucleus: Neuroanatomic landscape of the mouse "pontine micturition center".
Verstegen AMJ, Vanderhorst V, Gray PA, Zeidel ML, Geerling JC
The Journal of comparative neurology 2017 Jul 1;525(10):2287-2309
The Journal of comparative neurology 2017 Jul 1;525(10):2287-2309
HSD2 neurons in the hindbrain drive sodium appetite.
Jarvie BC, Palmiter RD
Nature neuroscience 2017 Feb;20(2):167-169
Nature neuroscience 2017 Feb;20(2):167-169
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human FoxP2 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line and human lung tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse FoxP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5647) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FoxP2 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.