Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AF5647 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse FoxP2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and mouse FoxP2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) FoxP1, rhFoxP3, and rhFoxP4 is observed.
- Reactivity
- Human, Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
O15409
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Chemosensory modulation of neural circuits for sodium appetite.
Efferent projections of excitatory and inhibitory preBötzinger Complex neurons.
Kölliker-Fuse GABAergic and glutamatergic neurons project to distinct targets.
Barrington's nucleus: Neuroanatomic landscape of the mouse "pontine micturition center".
HSD2 neurons in the hindbrain drive sodium appetite.
Lee S, Augustine V, Zhao Y, Ebisu H, Ho B, Kong D, Oka Y
Nature 2019 Apr;568(7750):93-97
Nature 2019 Apr;568(7750):93-97
Efferent projections of excitatory and inhibitory preBötzinger Complex neurons.
Yang CF, Feldman JL
The Journal of comparative neurology 2018 Jun 1;526(8):1389-1402
The Journal of comparative neurology 2018 Jun 1;526(8):1389-1402
Kölliker-Fuse GABAergic and glutamatergic neurons project to distinct targets.
Geerling JC, Yokota S, Rukhadze I, Roe D, Chamberlin NL
The Journal of comparative neurology 2017 Jun 1;525(8):1844-1860
The Journal of comparative neurology 2017 Jun 1;525(8):1844-1860
Barrington's nucleus: Neuroanatomic landscape of the mouse "pontine micturition center".
Verstegen AMJ, Vanderhorst V, Gray PA, Zeidel ML, Geerling JC
The Journal of comparative neurology 2017 Jul 1;525(10):2287-2309
The Journal of comparative neurology 2017 Jul 1;525(10):2287-2309
HSD2 neurons in the hindbrain drive sodium appetite.
Jarvie BC, Palmiter RD
Nature neuroscience 2017 Feb;20(2):167-169
Nature neuroscience 2017 Feb;20(2):167-169
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human FoxP2 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line and human lung tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse FoxP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5647) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FoxP2 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- FoxP2 in HeLa Human Cell Line. FoxP2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 10 µg/mL Sheep Anti-Human/Mouse FoxP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5647) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.