Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- MA5-24183 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TAFA5 Monoclonal Antibody (463102)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, no cross-reactivity with recombinant human TAFA2, 3, or 4 is observed. Reconstitute at 0.5 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 463102
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical analysis of TAFA5 in immersion fixed paraffin-embedded sections of human cerebellum. Samples were incubated in TAFA5 monoclonal antibody (Product # MA5-24183) using a dilution of 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining waslocalized to Purkinje neurons.