Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C498904 - Provider product page
- Provider
- LSBio
- Product name
- GARS / Glycyl tRNA Synthetase Antibody LS-C498904
- Antibody type
- Polyclonal
- Description
- Protein G purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating GARS in HL-60 whole cell lysate;Lane 1: Rabbit monoclonal IgG(1?g)instead of GARS Antibody in HL-60 whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000);Lane 2: GARS Antibody(8?g)+ HL-60 whole cell lysate(500?g);Lane 3: HL-60 whole cell lysate (20?g);
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating GARS in HL-60 whole cell lysate;Lane 1: Rabbit monoclonal IgG(1?g)instead of GARS Antibody in HL-60 whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000);Lane 2: GARS Antibody(8?g)+ HL-60 whole cell lysate(500?g);Lane 3: HL-60 whole cell lysate (20?g);
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue using GARS Antibody at dilution of 1:100