LS-C498904

antibody from LSBio

Targeting: GARS1 CMT2D, DSMAV, GARS, GlyRS, SMAD1

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C498904

Provider

LSBio

Product name

GARS / Glycyl tRNA Synthetase Antibody LS-C498904

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Storage

Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Positive WB detected in:THP-1 whole cell lysate,U87 whole cell lysate,HL-60 whole cell lysate,Mouse brain tissue,Mouse liver tissue,Rat brain tissue,Rat liver tissue;All lanes:GARS antibody at 3?g/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 84 KDa;Observed band size: 84,50 KDa;

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot Positive WB detected in: U87 whole cell lysate, Mouse brain tissue, Mouse liver tissue All lanes: GARS antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 84 kDa Observed band size: 84 kDa

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating GARS in HL-60 whole cell lysate;Lane 1: Rabbit monoclonal IgG(1?g)instead of GARS Antibody in HL-60 whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000);Lane 2: GARS Antibody(8?g)+ HL-60 whole cell lysate(500?g);Lane 3: HL-60 whole cell lysate (20?g);

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating GARS in HL-60 whole cell lysate;Lane 1: Rabbit monoclonal IgG(1?g)instead of GARS Antibody in HL-60 whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000);Lane 2: GARS Antibody(8?g)+ HL-60 whole cell lysate(500?g);Lane 3: HL-60 whole cell lysate (20?g);

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue using GARS Antibody at dilution of 1:100