15831-1-AP

antibody from Proteintech Group

Targeting: GARS1 CMT2D, DSMAV, GARS, GlyRS, SMAD1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

15831-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#15831-1-AP, RRID:AB_2294385

Product name

GARS antibody

Antibody type

Polyclonal

Description

KD/KO validated GARS antibody (Cat. #15831-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Glycyl-tRNA Synthetase Induces Psoriasis-Like Skin by Facilitating Skin Inflammation and Vascular Endothelial Cell Angiogenesis.

Zheng YX, Chen XB, Xu F, Cui YZ, Wang ZY, Zhou Y, Fu NC, Yang XY, Chen XY, Zheng M, Man XY
The Journal of investigative dermatology 2024 Apr;144(4):774-785.e10

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Comprehensive proteogenomic characterization of early duodenal cancer reveals the carcinogenesis tracks of different subtypes.

Li L, Jiang D, Liu H, Guo C, Zhao R, Zhang Q, Xu C, Qin Z, Feng J, Liu Y, Wang H, Chen W, Zhang X, Li B, Bai L, Tian S, Tan S, Yu Z, Chen L, Huang J, Zhao JY, Hou Y, Ding C
Nature communications 2023 Mar 29;14(1):1751

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Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules.

Cui Q, Bi H, Lv Z, Wu Q, Hua J, Gu B, Huo C, Tang M, Chen Y, Chen C, Chen S, Zhang X, Wu Z, Lao Z, Sheng N, Shen C, Zhang Y, Wu ZY, Jin Z, Yang P, Liu H, Li J, Bai G
Cell 2023 Feb 16;186(4):803-820.e25

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Integrated proteogenomic characterization of urothelial carcinoma of the bladder.

Xu N, Yao Z, Shang G, Ye D, Wang H, Zhang H, Qu Y, Xu F, Wang Y, Qin Z, Zhu J, Zhang F, Feng J, Tian S, Liu Y, Zhao J, Hou J, Guo J, Hou Y, Ding C
Journal of hematology & oncology 2022 Jun 3;15(1):76

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Immunoprecipation Assay to Quantify the Amount of tRNAs associated with Their Interacting Proteins in Tissue and Cell Culture.

Das S, Zuko A, Thompson R, Storkebaum E, Ignatova Z
Bio-protocol 2022 Feb 20;12(4):e4335

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SIRT2-knockdown rescues GARS-induced Charcot-Marie-Tooth neuropathy.

Zhao Y, Xie L, Shen C, Qi Q, Qin Y, Xing J, Zhou D, Qi Y, Yan Z, Lin X, Dai R, Lin J, Yu W
Aging cell 2021 Jun;20(6):e13391

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The tRNA-like small noncoding RNA mascRNA promotes global protein translation.

Lu X, Huang J, Wu S, Zheng Q, Liu P, Feng H, Su X, Fu H, Xi Q, Wang G
EMBO reports 2020 Dec 3;21(12):e49684

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HDAC6 is a therapeutic target in mutant GARS-induced Charcot-Marie-Tooth disease.

Benoy V, Van Helleputte L, Prior R, d'Ydewalle C, Haeck W, Geens N, Scheveneels W, Schevenels B, Cader MZ, Talbot K, Kozikowski AP, Vanden Berghe P, Van Damme P, Robberecht W, Van Den Bosch L
Brain : a journal of neurology 2018 Mar 1;141(3):673-687

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Mutations in glycyl-tRNA synthetase impair mitochondrial metabolism in neurons.

Boczonadi V, Meyer K, Gonczarowska-Jorge H, Griffin H, Roos A, Bartsakoulia M, Bansagi B, Ricci G, Palinkas F, Zahedi RP, Bruni F, Kaspar B, Lochmüller H, Boycott KM, Müller JS, Horvath R
Human molecular genetics 2018 Jun 15;27(12):2187-2204

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Impaired protein translation in Drosophila models for Charcot-Marie-Tooth neuropathy caused by mutant tRNA synthetases.

Niehues S, Bussmann J, Steffes G, Erdmann I, Köhrer C, Sun L, Wagner M, Schäfer K, Wang G, Koerdt SN, Stum M, Jaiswal S, RajBhandary UL, Thomas U, Aberle H, Burgess RW, Yang XL, Dieterich D, Storkebaum E
Nature communications 2015 Jul 3;6:7520

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

human brain tissue were subjected to SDS PAGE followed by western blot with 15831-1-AP(GARS antibody) at dilution of 1:500

Sample type

tissue

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using GARS antibody 15831-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human skin cancer using 15831-1-AP(GARS antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue