PA5-96322

antibody from Invitrogen Antibodies

Targeting: GARS1 CMT2D, DSMAV, GARS, GlyRS, SMAD1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96322

Provider

Invitrogen Antibodies

Product name

GARS Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: AEEVLAPLRL AVRQQGDLVR KLKEDKAPQV DVDKAVAELK ARKRVLEAKE LALQPKDDIV DRAKMEDTLK RRFFYDQAFA IYGGVSGLYD FGPVGCALKN NIIQTWRQHF IQEEQILEID CTMLTPEPVL KTSGHVDKFA DFMVKDVKNG ECFRADHLLK AHLQKLMSDK KCSVEKKSEM ESVLAQLDNY GQQELADLFV NYNVKSPITG NDLSPPVSFN LMFKTFIGPG GNMPGYLRPE TAQGIFLNFK RLLEFNQGKL PFAAAQIGNS FRNEISPRSG LIRVREFTMA EIEHFVDPSE KDHPKFQNVA D; Positive Samples: Jurkat, 293T, U-87MG, Raji, M21, HT-29, Mouse brain, Mouse spinal cord; Cellular Location: Cell projection, Cytoplasm, Mitochondrion, axon

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.6 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of GARS in extracts of various cell lines using GARS Polyclonal Antibody (Product # PA5-96322) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of GARS in extracts of various cell lines using GARS Polyclonal Antibody (Product # PA5-96322) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of GARS was performed in NIH/3T3 cells using GARS Polyclonal Antibody (Product # PA5-96322).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of GARS was performed in NIH-3T3 cells using GARS Polyclonal Antibody (Product # PA5-96322) at a dilution of 1:200. Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GARS in paraffin-embedded rat brain using GARS Polyclonal Antibody (Product # PA5-96322) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GARS in paraffin-embedded mouse brain using GARS Polyclonal Antibody (Product # PA5-96322) at a dilution of 1:200.