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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- PA5-144253 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GARS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 3 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GARS in U87 whole cell lysate, Mouse brain tissue, Mouse liver tissue using a dilution of 3 µg/mL. Samples were incubated with GARS polyclonal antibody (Product # PA5-144253). Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 84 kDa. Observed band size: 84 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of HepG2 cells using GARS polyclonal antibody (Product # PA5-144253) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of GARS in HL60 whole cell lysate; Lane 1: Rabbit control IgG. Samples were incubated with GARS polyclonal antibody (Product # PA5-144253).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded human testis tissue using GARS polyclonal antibody (Product # PA5-144253) at dilution of 1:100.
