Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PA1597 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-HDJ2/DNAJA1 Antibody
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for HDJ2/DNAJA1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, ICC/IF. Reactive species: Human;Mouse;Rat. HDJ2/DNAJA1 information: Molecular Weight: 44868 MW; Subcellular Localization: Membrane ; Lipid-anchor . Cytoplasm . Microsome . Nucleus . Cytoplasm, perinuclear region . Mitochondrion . Primarily associated with microsomes. A minor proportion is associated with mitochondria (By similarity). Primarily cytoplasmic. A minor proportion is associated with nuclei; Tissue Specificity: Ubiquitous. Isoform 2 is highly expressed in testis and lung, but detected at low levels in thymus, prostate, colon and liver.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references DnaJ homolog subfamily A member1 (DnaJ1) is a newly discovered anti-apoptotic protein regulated by azadirachtin in Sf9 cells.
Shu B, Jia J, Zhang J, Sethuraman V, Yi X, Zhong G
BMC genomics 2018 May 29;19(1):413
BMC genomics 2018 May 29;19(1):413
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Anti-HDJ2 antibody, PA1597, Western blottingAll lanes: Anti HDJ2 (PA1597) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Lung Tissue Lysate at 50ugLane 3: Mouse Brain Tissue Lysate at 50ugLane 4: Mouse Lung Tissue Lysate at 50ugLane 5: U87 Whole Cell Lysate at 40ugLane 6: A549 Whole Cell Lysate at 40ugLane 7: COLO320 Whole Cell Lysate at 40ugLane 8: A431 Whole Cell Lysate at 40ugLane 9: HT1080 Whole Cell Lysate at 40ugPredicted bind size: 45KDObserved bind size: 45KD
- Additional image