PA5-36611

antibody from Invitrogen Antibodies

Targeting: SCAF4 DKFZp434E098, KIAA1172, SFRS15, SRA4

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-36611

Provider

Invitrogen Antibodies

Product name

SFRS15 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody detects endogenous protein at a molecular weight of 126 kDa. Purity is >95% by SDS-PAGE.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Large-scale and high-resolution mass spectrometry-based proteomics profiling defines molecular subtypes of esophageal cancer for therapeutic targeting.

Liu W, Xie L, He YH, Wu ZY, Liu LX, Bai XF, Deng DX, Xu XE, Liao LD, Lin W, Heng JH, Xu X, Peng L, Huang QF, Li CY, Zhang ZD, Wang W, Zhang GR, Gao X, Wang SH, Li CQ, Xu LY, Liu W, Li EM
Nature communications 2021 Aug 16;12(1):4961

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 4 Subtype diagnostic signature composed of ELOA and SCAF4 were identified in EC. a Bar plots of the frequency of the signatures in 100 times feature selections. Red, green, cyan, and purple indicate that the maximum number of features is 1, 2, 3, and 4, respectively. b Box plots of the cross-validation AUCs (area under the ROC curve, n = 100) of the 11 signatures shown. c Box plots of log 2 -transformed protein expression ratios of ELOA (left) and SCAF4 (right) (S1, n = 61; S2, n = 63). P values are calculated by the two-sided Wilcoxon rank-sum test. d ROC curve of the SVM model with signature 4 (ELOA, SCAF4). e Representative IHC (immunohistochemistry) images of ELOA and SCAF4 protein expression in the independent EC Cohort (Cohort 2, n = 295). Scale bars, 100 um. f Box plots of IHC scores of ELOA (left) and SCAF4 (right) in the predicted S1 and S2 patients in Cohort 2 (S1, n = 97; S2, n = 198). P values are calculated by the two-sided Wilcoxon rank-sum test. g , h Kaplan-Meier curves of OS ( g ) and DFS ( h ) for each predicted subtype in the independent EC Cohort 2. P values are calculated by two-sided log-rank test. In the box plots b , c , f ), the middle bar represents the median, and the box represents the interquartile range; bars extend to 1.5x the interquartile range.